Search for dissertations about: "diagnostic assay"

Showing result 1 - 5 of 134 swedish dissertations containing the words diagnostic assay.

  1. 1. Tethered Lipid Vesicles as Nanoscale Sensor Elements

    Author : Seyed Tabaei; [2012]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; aquaglyceroporin; lipid vesicles; total internal reflection fluorescence TIRF microscopy; cell membrane; aquaporin; pore formation; surface plasmon resonance SPR ; phospholipase A2; antiviral peptide; lipid bilayer; biosensing; microfluidics;

    Abstract : The primary aim of this thesis work has been to explore and advance the potential of surface tethered lipid vesicles as a platform for studying in particular the following three biomembrane-related processes: trans-membrane transport, membrane-peptide interactions and the activity of membrane active enzymes. First, a method based on surface plasmon resonance (SPR) spectroscopy was developed for studying passive diffusion processes as well as the membrane-protein mediated transport of small non electrolyte molecules across lipid bilayers. READ MORE

  2. 2. Novel diagnostic microarray assay formats towards comprehensive on-site analysis

    University dissertation from Stockholm : KTH

    Author : Jesper Gantelius; Helene Andersson-Svahn; Christer Wingren; [2009]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; magnetic microbeads; gold nanobeads; microarrays; lateral flow; on-site diagnosis; SNPs; pestivirus; autoimmunity; contagious bovine pleuropneumonia; comsol multiphysics; finite element method; NATURAL SCIENCES Biology Organism biology Microbiology; NATURVETENSKAP Biologi Organismbiologi Mikrobiologi; MEDICINE Chemistry Biochemistry; MEDICIN Kemi Biokemi;

    Abstract : Advances in molecular methods for analyzing DNA, RNA and proteins in humans as well as in other animals, plants, fungi, bacteria or viruses have greatly increased the resolution with which we can study life’s complexity and dynamics on earth. While genomic, transcriptomic and proteomic laboratory tools for molecular diagnosis of disease are rapidly becoming more comprehensive, the access to such advanced yet often expensive and centralized procedures is limited. READ MORE

  3. 3. Diagnostic and prognostic markers in sepsis

    University dissertation from Stockholm : Karolinska Institutet, Dept of Medicine, Huddinge

    Author : Patrik Gille-Johnson; Karolinska Institutet.; Karolinska Institutet.; [2013]

    Abstract : Sepsis is a life-threatening disease affecting millions of people globally. The more severe forms are considered to be a consequence of an unbalanced systemic inflammatory response to infection, causing organ dysfunction, vascular leakage and hypotension. An early diagnosis followed by appropriate antimicrobial therapy is critical for the outcome. READ MORE

  4. 4. Development of Enhanced Molecular Diagnostic Tools for Protein Detection and Analysis

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Tonge Ebai; Ulf Landegren; Masood Kamali-Moghaddam; Peter Nilsson; [2017]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; protein detection; proximity ligation assays; proximity extension assay; rolling circle amplification; ELISA; flow cytometry; fluorescence microscopy;

    Abstract : Improved diagnosis, prognosis and disease follow-up is a fundamental procedure and a constant challenge in medicine.  Among the different molecular biomarkers, proteins are the essential regulatory component in blood; hence, by developing enhanced specific and sensitive molecular tools will gives great insight into the different processes in disease treatment. READ MORE

  5. 5. Diagnostic PCR for the Detection of Yersinia enterocolitica and Salmonella in the Food-Chain: Reliability of PCR Performance

    University dissertation from Applied Microbiology (LTH)

    Author : Rickard Knutsson; [2001]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Livsmedelsteknik; Food and drink technology; Kemiteknik och kemisk teknologi; Chemical technology and engineering; real-time PCR; YPCE medium; Enrichment PCR; DNA polymerases; PCR inhibitors; pre-PCR processing; Salmonella; Diagnostic PCR; Yersinia enterocolitica; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Abstract : This thesis deals with the methodological advances of diagnostic PCR including reliability of PCR and pre-PCR processing of food and feed samples. Diagnostic PCR has been greatly improved by the introduction of the second generation of PCR, so-called real-time PCR. READ MORE