Search for dissertations about: "diagnostic assay"

Showing result 1 - 5 of 156 swedish dissertations containing the words diagnostic assay.

2. 1. Novel diagnostic microarray assay formats towards comprehensive on-site analysis

   Author : Jesper Gantelius; Helene Andersson-Svahn; Christer Wingren; KTH; []
   Keywords : NATURAL SCIENCES; NATURVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; NATURVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; NATURAL SCIENCES; MEDICAL AND HEALTH SCIENCES; magnetic microbeads; gold nanobeads; microarrays; lateral flow; on-site diagnosis; SNPs; pestivirus; autoimmunity; contagious bovine pleuropneumonia; comsol multiphysics; finite element method; Microbiology; Mikrobiologi; Biochemistry; Biokemi;

   Abstract : Advances in molecular methods for analyzing DNA, RNA and proteins in humans as well as in other animals, plants, fungi, bacteria or viruses have greatly increased the resolution with which we can study life’s complexity and dynamics on earth. While genomic, transcriptomic and proteomic laboratory tools for molecular diagnosis of disease are rapidly becoming more comprehensive, the access to such advanced yet often expensive and centralized procedures is limited. READ MORE

4. 2. Diagnostic and prognostic markers in sepsis

   Author : Patrik Gille-Johnson; Karolinska Institutet; Karolinska Institutet; []
   Keywords : ;

   Abstract : Sepsis is a life-threatening disease affecting millions of people globally. The more severe forms are considered to be a consequence of an unbalanced systemic inflammatory response to infection, causing organ dysfunction, vascular leakage and hypotension. An early diagnosis followed by appropriate antimicrobial therapy is critical for the outcome. READ MORE

5. 3. Development of Enhanced Molecular Diagnostic Tools for Protein Detection and Analysis

   Author : Tonge Ebai; Ulf Landegren; Masood Kamali-Moghaddam; Peter Nilsson; Uppsala universitet; []
   Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; protein detection; proximity ligation assays; proximity extension assay; rolling circle amplification; ELISA; flow cytometry; fluorescence microscopy;

   Abstract : Improved diagnosis, prognosis and disease follow-up is a fundamental procedure and a constant challenge in medicine.  Among the different molecular biomarkers, proteins are the essential regulatory component in blood; hence, by developing enhanced specific and sensitive molecular tools will gives great insight into the different processes in disease treatment. READ MORE

6. 4. Diagnostic PCR for the Detection of Yersinia enterocolitica and Salmonella in the Food-Chain: Reliability of PCR Performance

   Author : Rickard Knutsson; Teknisk mikrobiologi; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Livsmedelsteknik; Food and drink technology; Kemiteknik och kemisk teknologi; Chemical technology and engineering; real-time PCR; YPCE medium; Enrichment PCR; DNA polymerases; PCR inhibitors; pre-PCR processing; Salmonella; Diagnostic PCR; Yersinia enterocolitica; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

   Abstract : This thesis deals with the methodological advances of diagnostic PCR including reliability of PCR and pre-PCR processing of food and feed samples. Diagnostic PCR has been greatly improved by the introduction of the second generation of PCR, so-called real-time PCR. READ MORE

7. 5. Development and Application of Proximity Assays for Proteome Analysis in Medicine

   Author : Felipe Marques Souza de Oliveira; Masood Kamali-Moghaddam; Ulf Landegren; Mats Gullberg; Janne Lehtiö; Uppsala universitet; []
   Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Solid-phase proximity ligation assay; post-translational modifications; glycosylation; phosphorylation; Enzyme-linked immunosorbent assay; immunoassay and rolling circle amplification; Proximity Extension Assay; inflammation protein biomarkers; autoantibodies; autoimmune disease;

   Abstract : Along with proteins, a myriad of different molecular biomarkers, such as post-translational modifications and autoantibodies, could be used in an attempt to improve disease detection and progression. In this thesis, I build on several iterations of the proximity ligation assay to develop and apply new adaptable methods to facilitate detection of proteins, autoantibodies and post-translational modifications. READ MORE