Search for dissertations about: "digital PCR"

Showing result 6 - 10 of 99 swedish dissertations containing the words digital PCR.

  2. 6. Quantitative detection of bacterial DNA in whole blood in bloodstream infection

    Author : Ingrid Ziegler; Kristoffer Strålin; Paula Mölling; Magnus Rasmussen; Örebro universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bloodstream infection; bacteremia; sepsis; DNA load; quantitative PCR; droplet digital PCR; 16S rDNA;

    Abstract : This thesis aims to increase the knowledge on how quantitative PCR can be used in the diagnostics of bloodstream infections, with an emphasis on quantitative elements.In Papers I and II, we evaluated quantitative data from two commercial PCR tests for pathogen detection directly in blood, Magicplex Sepsis (I) and SeptiFast (II), from patients with suspected sepsis. READ MORE

  4. 7. Dispersal of ticks and their microorganisms by African-Western Palaearctic migratory birds

    Author : Tove Hoffman; Björn Olsen; Åke Lundkvist; Kenneth Nilsson; Johan Normark; Uppsala universitet; []
    Keywords : African-Western Palaearctic; migratory birds; Ixodidae; Hyalomma rufipes; Ixodes; Alkhurma hemorrhagic fever virus; Anaplasma phagocytophilum; Francisella; Midichloria; Rickettsia aeschlimannii; PCR; microfluidic qPCR; metagenomics; Biologi; Biology;

    Abstract : In Europe, tick-borne diseases are the most widespread and common vector-borne diseases and their geographical distribution is increasing. The dispersal of ticks depends on the movements of their vertebrate hosts. Avian hosts are more likely to be involved in long-distance range expansion of ticks due to their migration pattern. READ MORE

  5. 8. Microfluidic and Molecular Tools for Genetic Analyses

    Author : Henrik Johansson; Mats Nilsson; Ivo Gut; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; microfluidics; PCR; sequencing; cancer genetics; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Medical Genetics; Medicinsk genetik;

    Abstract : Methods that enable interrogation of multiple genomic regions in parallel are very useful for efficient detection of genetic variation. Two different types of probes are described in this thesis that can be used for direct analysis or for sample preparation upstream of Next Generation Sequencing. READ MORE

  6. 9. Spotted Fever Rickettsioses in Sweden : Aspects of Epidemiology, Clinical Manifestations and Co-infections

    Author : Anders Lindblom; Kenneth Nilsson; Björn Olsen; Björn Herrmann; Clas Ahlm; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Rickettsia helvetica; Rickettsia felis; co-infection; erythema migrans; meningitis; serology; PCR; western blot; Infektionssjukdomar; Infectious Diseases;

    Abstract : The spotted fever group rickettsiae are emerging diseases. They cause damage in their hosts by invading the endothelium in small to medium-sized blood vessels, which results in vasculitis that can cause clinical manifestations from most organs. READ MORE

  7. 10. The Impact of Nandrolone Decanoate on Neuropeptidergic Mechanisms Related to Cognition, Aggression, Reward and Dependence

    Author : Kristina Magnusson; Fred Nyberg; Mathias Hallberg; Santi Mario Spampinato; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Anabolic androgenic steroids; Nandrolone decanoate; Substance P; Dynorphin A; KOP receptor; Prodynorphin; Radioimmunoassay; Autoradiography; Morris water maze; PCR; Central nervous system; Biological research on drug dependence; Biologisk beroendeforskning; Biological Research on Drug Dependence; Biologisk beroendeforskning;

    Abstract : The abuse of anabolic androgenic steroids (AAS) is becoming increasingly common and may result in a range of physiological as well as psychological effects such as altered behavior in terms of increased aggression, cognitive dysfunction and addictive behavior. AAS comprise testosterone and its derivatives, of which nandrolone is one of the more common. READ MORE