Search for dissertations about: "directed evolution"

Showing result 1 - 5 of 102 swedish dissertations containing the words directed evolution.

  1. 1. Directed Evolution of Glutathione Transferases with Altered Substrate Selectivity Profiles : A Laboratory Evolution Study Shedding Light on the Multidimensional Nature of Epistasis

    Author : Wei Zhang; Bengt Mannervik; Bengt-Harald Jonsson; Uppsala universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; glutathione transferase; azathioprine; directed evolution; semi-rational design; catalytic mechanism; saturation mutagenesis; reduced amino acid alphabets; molecular docking; protein evolution; multivariate data analysis; epistasis; fitness landscape; evolutionary trajectories; Biochemistry; Biokemi; Biokemi; Biochemistry;

    Abstract : Directed evolution is generally regarded as a useful approach in protein engineering. By subjecting members of a mutant library to the power of Darwinian evolution, desired protein properties are obtained. Numerous reports have appeared in the literature showing the success of tailoring proteins for various applications by this method. READ MORE

  2. 2. Oxidation of 1,2-Diols Using Alcohol Dehydrogenases : From Kinetic Characterization to Directed Evolution

    Author : Cecilia Blikstad; Mikael Widersten; Stefan Lutz; Uppsala universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; enzyme kinetics; alcohol dehydrogenase; directed evolution; enzyme engineering; diol; α-hydroxy aldehyde; Biokemi; Biochemistry;

    Abstract : The use of enzymes as catalysts for chemical transformations has emerged as a “greener” alternative to traditional organic synthesis. An issue to solve though, is that enzymes are designed by nature to catalyze reactions in a living cell and therefore, in many cases, do not meet the requirements of a suitable biocatalyst. READ MORE

  3. 3. Computational Protein Evolution : Modeling the Selectivity and Promiscuity of Engineered Enzymes

    Author : Klaudia Szeler; Shina C. Lynn Kamerlin; Mikael Widersten; Maria Ramos; Uppsala universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; enzymes; kinetics; catalysis; density functional theory; empirical valence bond; diesters; evolution; promiscuity; Biokemi; Biochemistry;

    Abstract : Enzymes are biological catalysts that significantly increase the rate of all biochemical reactions that take place within cells and are essential to maintain life. Many questions regarding their function remain unknown. READ MORE

  4. 4. Droplet microfluidics for directed evolution of biocatalysts

    Author : Staffan Sjöström; Helene Andersson Svahn; Florian Hollfelder; KTH; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Droplet microfluidics; Enzymes; directed evolution; high throughput screening; Biotechnology; Bioteknologi;

    Abstract : Biocatalysts, biologically derived catalysts, are of great importance for a wide range of industrial applications. They are used in the production of for example foods, pharmaceuticals and biofuels. Improving biocatalysts commonly relies on directed evolution, i.e. READ MORE

  5. 5. Aldolases for Enzymatic Carboligation : Directed Evolution and Enzyme Structure-Function Relationship Studies

    Author : Huan Ma; Mikael Widersten; Wolf-Dieter Fessner; Uppsala universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; aldolase; 2-deoxyribose-5-phosphate aldolase; fructose-6-phosphate aldolase; directed evolution; enzyme dynamics; Biokemi; Biochemistry;

    Abstract : The research summarized in this thesis focuses on directed evolution and enzyme mechanism studies of two aldolases: 2-deoxyribose-5-phosphate aldolase (DERA) and fructose-6-phosphate aldolase (FSA).Aldolases are nature’s own catalysts for one of the most fundamental reactions in organic chemistry: the formation of new carbon-carbon bonds. READ MORE