Search for dissertations about: "directed evolution"

Showing result 6 - 10 of 108 swedish dissertations containing the words directed evolution.

  2. 6. Directed Enzyme Evolution of Theta Class Glutathione Transferase : Studies of Recombinant Libraries and Enhancement of Activity toward the Anticancer Drug 1,3-bis(2-Chloroethyl)-1-nitrosourea

    Author : Anna-Karin Larsson; Bengt Mannervik; Ian Cotgreave; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; glutathione transferase; directed evolution; 1; 3-bis 2-chloroethyl -1-nitrosourea; alkyltransferase; recombinant libraries; chloroethylnitrosourea; multi-dimensional data analysis; Biokemi; Biochemistry; Biokemi;

    Abstract : Glutathione transferases (GSTs) are detoxication enzymes involved in the cellular protection against a wide range of reactive substances. The role of GSTs is to catalyze the conjugation of glutathione with electrophilic compounds, which generally results in less toxic products. READ MORE

  4. 7. Protein Engineering by Directed Evolution and Rational Design

    Author : Torbjörn Gräslund; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; ion-exchange chromatography; subtilisin; Klenow DNA polymerase; Taq DNA polymerase; directed evolution; rational design; charge engineering; phosphonylating inhibitor; phage display; circular dichroism; protein A; Bioengineering; Bioteknik;

    Abstract : .... READ MORE

  5. 8. Characterization and Directed Evolution of an Alcohol Dehydrogenase : A Study Towards Understanding of Three Central Aspects of Substrate Selectivity

    Author : Emil Hamnevik; Mikael Widersten; Gerrit Poelarends; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Alcohol dehydrogenase; ADH-A; Biocatalysis; Directed evolution; Enantioselectivity; Enzyme kinetics; Enzyme mechanisms; Protein Engineering; Regioselectivity; Substrate selectivity; Biokemi; Biochemistry;

    Abstract : Many different chemicals are used in the everyday life, like detergents and pharmaceuticals. However, their production has a big impact on health and environment as much of the raw materials are not renewable and the standard ways of production in many cases includes toxic and environmentally hazardous components. READ MORE

  6. 9. Staphylococcal surface display in directed evolution

    Author : Nina Kronqvist; Stefan Ståhl; Mats A. A. Persson; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; combinatorial library; directed evoluation; Gram-positive bacteria. protein engineering; Bioengineering; Bioteknik;

    Abstract : Engineered affinity proteins have together with naturally derived antibodies becomeindispensable tools in many areas of life-science and with the increasing number ofapplications, the need for high-throughput methods for generation of such different affinityproteins is evident. Today, combinatorial protein engineering is the most successful strategy toisolate novel non-immunoglobulin affinity proteins. READ MORE

  7. 10. Dynamics and Mechanisms of Adaptive Evolution in Bacteria

    Author : Song Sun; Dan Andersson; Diarmaid Hughes; Josep Casadesús; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; adaptive evolution; mutation; genome rearrangements; antibiotic resistance; gene amplification; genome reduction; directed evolution; Biologi med inriktning mot mikrobiologi; Biology with specialization in Microbiology;

    Abstract : Determining the properties of mutations is fundamental to understanding the mechanisms of adaptive evolution. The major goal of this thesis is to investigate the mechanisms of bacterial adaptation to new environments using experimental evolution. READ MORE