Search for dissertations about: "dna detection methods"

Showing result 16 - 20 of 200 swedish dissertations containing the words dna detection methods.

2. 16. Single-stranded DNA : methods and application in nanotechnology

   Author : Giulio Bernardinelli; Karolinska Institutet; Karolinska Institutet; []
   Keywords : ;

   Abstract : Basic molecular and cell research, production of recombinant proteins, diagnostic detection of genetic mutations, construction of nanostructures and high-throughput DNA sequencing are only a few examples of the diverse set of applications that are amenable thanks to the availability of synthetic DNA polymers in biomedicine. Strategic investments and technical progress together with the introduction of automation in the synthesis of DNA oligomers enabled to transform, in just a few decades, a process mastered only by a niche of biochemists into an affordable and available custom-made product to every scientific field. READ MORE

4. 17. Detection and epidemiologic subtyping of Legionella pneumophilia using DNA-based molecular methods

   Author : Sverker Bernander; Karolinska Institutet; Karolinska Institutet; []
   Keywords : Legionella; AFLP; PFGE; Nested PCR; lag-1; hospital; outbreaks;

   Abstract : Legionella pneumophila causes 2 - 6 % of hospitalised pneumonia cases. Several community- and hospital-acquired outbreaks have been reported over the years. Legionnaires' disease (LD) or legionellosis is also a frequent cause of pneumonia amongst travellers. READ MORE

5. 18. Detection of Bio-analytes with Streaming Current : From Fundamental Principles to Novel Applications

   Author : Siddharth S. Sahu; Apurba Dev; Shi-Li Zhang; Michel Calame; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Streaming current; Electrokinetic biosensor; Size and Charge effects; membrane protein profiling of extracellular vesicles; sandwich immunoassay; charge-labelled antibodies; multiplexed detection; exosome heterogeneity;

   Abstract : A biosensor based on streaming current is a new and relatively unexplored subject with significant potential. This thesis attempts to gain a deeper understanding of the governing principles, and then exploit them to further improve its performance as well as develop novel applications. READ MORE

6. 19. Proximity Ligation : Transforming protein analysis into nucleic acid detection through proximity-dependent ligation of DNA sequence tagged protein-binders

   Author : Simon Fredriksson; Larry Gold; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Proximity ligation; proteomics; SELEX; antibody; DNA microarray; Molekylärmedicin; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Molecular Medicine; molekylär medicin genetik och patologi ;

   Abstract : A novel technology for protein detection, proximity ligation, has been developed along with improved methods for in situ synthesis of DNA microarrays. Proximity ligation enables a specific and quantitative transformation of proteins present in a sample into nucleic acid sequences. READ MORE

7. 20. Bayesian inference for detection problems in biology

   Author : Ronny Hedell; Göteborgs universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; PCR; Bayesian inference; Forensic DNA analysis; Syndromic surveillance; Bacillus anthracis; Markov chain Monte Carlo; Allele dropout; Bacillus anthracis;

   Abstract : This thesis is about different kinds of detection problems in biology: detection of DNA sequences in crime scene samples, detection of harmful bacteria in feed and food stuff and detection of epidemical diseases in animal populations. In each case, biological data is produced or collected in order to determine which DNA sequences, bacteria types or diseases are present, if any. READ MORE