Search for dissertations about: "dna repair comet assay"

Showing result 1 - 5 of 12 swedish dissertations containing the words dna repair comet assay.

  2. 1. Chemically Induced DNA Damage in Extended-term Cultures of Human Lymphocytes

    Author : Maria Andersson; Björn Hellman; Leif Busk; Uppsala universitet; []
    Keywords : Toxicology; Comet assay; DNA-damaging agents; DNA repair; Extended-term Cultures of Human Lymphocytes; Genotoxicity; Induced DNA damage; In vitro; Mouse Lymphoma Cells; Primary Cell Cultures; Reactive Oxygen Species; Toxikologi;

    Abstract : Generation of DNA damage is regarded to be an important initial event in the development cancer. Consequently, a battery of tests have been developed to detect different types of genotoxic effects in order to be able to predict the potential genotoxicity and mutagenicity of chemicals, including both pharmaceutical drugs and various types of environmental and occupational agents, as well as dietary factors. READ MORE

  4. 2. DNA repair and genome organisation in mammalian cells : studies involving single-cell electrophoresis

    Author : Stefan Eriksson; Bert van Zeeland; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Physical Biology; fysikalisk biologi;

    Abstract : The comet assay (single-cell electrophoresis) is a comparably new method for measurement of DNA strand breakage, primarily in eukaryotic cells. Its main advantage is that it works on the single-cell level, making it possible to detect heterogeneity in DNA damage induction and repair. READ MORE

  5. 3. Application of Padlock Probe Based Nucleic Acid Analysis In Situ

    Author : Sara Henriksson; Mats Nilsson; Ulf Landegren; Harvest Gu; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Padlock probe; rolling circle amplification; single cell gel electrophoresis assay; comet assay; Crimean Congo hemorrhagic fever virus; Molecular medicine; Molekylär medicin; Molekylär medicin; Molecular Medicine;

    Abstract : The great variation displayed by nucleic acid molecules in human cells, and the continuous discovery of their impact on life, consequently require continuous refinements of molecular analysis techniques. Padlock probes and rolling circle amplification offer single nucleotide discrimination in situ, a high signal-to-noise ratio and localized detection within cells and tissues. READ MORE

  6. 4. Influence of genetic polymorphisms on DNA repair, p53 mutations and cancer risk

    Author : Charlotta Ryk; Karolinska Institutet; Karolinska Institutet; []
    Keywords : polymorphisms; metabolism; DNA repair; p53; lung cancer; bladder cancer;

    Abstract : Individuals have different susceptibility towards environmental exposures that may cause or contribute to disease. This inter-individual variation is partly due to genetic polymorphisms in genes involved in DNA repair and metabolism, which have potential to modulate the function of the encoded proteins. READ MORE

  7. 5. Radiosensitivity in lung cancer with focus on p53

    Author : Michael Bergqvist; Claes Mercke; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Oncology; Lung cancer; comet assay; p53; cDNA; p53 antibodies; Lungcancer; Komet metoden; p53 antikroppar; Onkologi; Oncology; Onkologi; Oncology; onkologi;

    Abstract : In Sweden approximately 2800 new lung cancer patients are diagnosed every year. Radiotherapy is used with curative intention in certain groups of patients. The aim of this thesis is to study the basis of differences in radioresistance and the possibility to predict response to radiotherapy. READ MORE