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Showing result 1 - 5 of 27 swedish dissertations matching the above criteria.

  2. 1. Cellulose hydrolysis by Trichoderma reesei cellulases: Studies on adsorption, sugar production and synergism of cellobiohydrolase I, II and endoglucanase II

    Author : Jozsef Medve; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; FPLC; cellulose hydrolysis; Trichoderma reesei; cellulase; cellobiohydrolase; endoglucanase; adsorption isotherm; synergism; enzyme purification; isoenzymes; quantitative analysis; Biochemistry; Metabolism; Biokemi;

    Abstract : Three major cellulases, cellobiohydrolase I and II (CBH I and II) and endoglucanase II (EG II) of Trichoderma reesei have been purified by ion-exchange chromatography in an FPLC system. Microcrystalline cellulose (Avicel) was hydrolysed by the single enzymes and by equimolar mixtures of CBH I-CBH II and CBH I-EG II. READ MORE

  4. 2. Hydrolytic and Oxidative Mechanisms Involved in Cellulose Degradation

    Author : Anu Nutt; Jerry Ståhlberg; Henrik Stålbrand; Uppsala universitet; []
    Keywords : Biochemistry; cellobiohydrolase; cellulase; cellulose; cellobiose dehydrogenase; endoglucanase; kinetics; synergism; competitive binding; Biokemi;

    Abstract : The enzymatic degradation of cellulose is an important process in nature. This thesis has focused on the degradation of cellulose by enzymes from two cellulose-degrading fungi, Hypocrea jecorina and Phanerochaete chrysosporium, including both the action of the individual enzymes and their synergistic interplay. READ MORE

  5. 3. Glycoside hydrolases from Rhodothermus marinus Modular organisation and structure-function relationships

    Author : Maher Abou-Hachem; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; calcium-binding; modular; endoglucanase; thermostable; CBM; xylanase; Biotechnology; Bioteknik;

    Abstract : The thermophilic bacterium Rhodothermus marinus produces several thermostable glycoside hydrolases. The studies presented in this thesis were performed on two enzymes, belonging to glycoside hydrolase families 10 and 12, produced by this microorganism. The family 10 xylanase, Xyn10A, is modular in architecture consisting of five domains or modules. READ MORE

  6. 4. Hydrophobic Fusion Tags: Implications For Bioseparation and Cellular Expression

    Author : Anna Collén; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; protein production; secretion; aqueous two-phase systems; Trichoderma reesei; endoglucanase; Biochemistry; Metabolism; Biokemi; metabolism;

    Abstract : The studies in this thesis have shown that the partitioning of endoglucanase I (EGI, Cel7B) from T. reesei could be significantly improved by relatively minor genetic engineering. By adding short peptides composed of tryptophan and proline residues to EGI, extreme partitioning could be obtained. READ MORE

  7. 5. Structural Studies of Three Glycosidases

    Author : Anna Larsson; Alwyn Jones; Janet Smith; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; glycosidase; dextranase; mannanase; endoglucanase; SeMet; MAD; crystal structure; Cell- och molekylärbiologi; Cell and molecular biology; Cell- och molekylärbiologi;

    Abstract : Glycosidases hydrolyse the glycosidic bond in carbohydrates. Structural studies of three glycosidases with different substrate specificities are presented in this work.Dextranase catalyzes the hydrolysis of α-1,6-glycosidic linkage in dextran polymers. READ MORE