Search for dissertations about: "enzyme immobilisation"

Showing result 1 - 5 of 17 swedish dissertations containing the words enzyme immobilisation.

  1. 1. Enzymatic synthesis of acrylates. Catalyst properties and development of process and product

    Author : Mathias Nordblad; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; enzyme kinetics; process development; enzyme stability; enzyme immobilisation; acrylation; lipase;

    Abstract : The increasingly apparent environmental problems in our surroundings necessitate a quest for sustainable development in all areas of human endeavour. Within the field of chemistry and the production of chemicals, the focus is increasingly on the development of green chemistry. READ MORE

  2. 2. Enzyme-Based Amperometric Biosensors in Flow-Systems for Biomolecule Detection

    Author : Niklas Larsson; Centrum för analys och syntes; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Microdialysis; Liquid chromatography; Flow injection; Polyelectrolytes; Carbohydrate; Mediator; Dehydrogenase; Peroxidase; Oxidase; Enzyme; Amperometric detection; Carbon paste; Biosensor; Graphite electrode; On-line monitoring; Analytical chemistry; Analytisk kemi;

    Abstract : Amperometric enzyme based biosensors were designed, developed and characterised in flow systems. Solid graphite and carbon paste electrodes were used as support for enzyme immobilisation. Both mediated and direct electron transfers between redox enzymes and electrodes were utilised for biosensor construction, as well as coupled enzyme systems. READ MORE

  3. 3. Enzymatic Synthesis of Alkyl Glycosides. New Applications for Glycoside Hydrolases

    Author : David Svensson; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; CGTase; cyclodextrin glycosyltransferase; alkyl glycoside; enzyme stabliity; enzymatic synthesis; enzyme immobilisation; surfactant; glycosylation;

    Abstract : The environmental problems caused by human interventions, such as the consumption of non-renewable resources and the emission of greenhouse gases are well-recognised. Calls have been made for immediate measures to develop more sustainable society worldwide. READ MORE

  4. 4. Lipases as Biocatalysts in Organic Media. Influence of Enzyme Preparation and Reaction Conditions on Activity and Selectivity

    Author : Mattias Persson; Avdelningen för klinisk kemi och farmakologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biochemical technology; Bioteknik; Biotechnology; water activity; temperature; enantioselectivity; immobilisation; organic solvents; lyophilisation; Lipases; Accurel EP-100; Biokemisk teknik; Proteins; enzymology; Proteiner; enzymologi;

    Abstract : The discovery that enzymes can function in organic solvents has broadened the scope of biocatalysis, making enzymes highly useful tools for organic chemists. The widespread use of enzymes as catalysts in organic solvents has, however, been limited by the low activity obtained for enzymes in organic solvents compared to aqueous solutions. READ MORE

  5. 5. Tools in biocatalysis : enzyme immobilisation on silica and synthesis of enantiopure amines

    Author : Karim Engelmark Cassimjee; Per Berglund; Torkel Berglund; Torbjörn Gräslund; KTH; []

    Abstract : This thesis presents two techniques in the field of biocatalysis: An enzyme immobilisation method based on the His6-tag for attachment on modified silica oxide beads, and it’s employment in aqueous and organic medium for synthesis applications. The method functions as a one step extraction and immobilisation protocol. READ MORE