Search for dissertations about: "enzyme libraries"

Showing result 1 - 5 of 31 swedish dissertations containing the words enzyme libraries.

  2. 1. Engineering Candida antarctica Lipase A for Enantioselective Transformations in Organic Synthesis : Design, Immobilization and Organic Solvent Screening of Smart Enzyme Libraries

    Author : Ylva Wikmark; Jan-Erling Bäckvall; Romas Kazlauskas; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Candida antarctica Lipase A; protein engineering; enzyme libraries; enzyme immobilization; biocatalysis; organisk kemi; Organic Chemistry;

    Abstract : The use of enzymes as catalysts in organic synthesis constitutes an attractive alternative to conventional chemical catalysis. Enzymes are non-toxic and biodegradable and they can operate under mild reaction conditions. READ MORE

  4. 2. Combinatorial protein engineering applied to enzyme catalysis and molecular recognition

    Author : Malin Eklund; KTH; []
    Keywords : phage display; selection; enzyme; affinity domains; crystal structure; affibody; lipase; protein engineering; protein A; assembly;

    Abstract : The recent development of methods for constructing andhandling large collections (libraries) of proteins, from whichvariants with desired traits can be isolated, hasrevolutionized the field of protein engineering. Key elementsof such methods are the various ways in which the genotypes(the genes) and the phenotypes (the encoded proteins) arephysically linked during the process. READ MORE

  5. 3. Directed Enzyme Evolution of Theta Class Glutathione Transferase : Studies of Recombinant Libraries and Enhancement of Activity toward the Anticancer Drug 1,3-bis(2-Chloroethyl)-1-nitrosourea

    Author : Anna-Karin Larsson; Bengt Mannervik; Ian Cotgreave; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; glutathione transferase; directed evolution; 1; 3-bis 2-chloroethyl -1-nitrosourea; alkyltransferase; recombinant libraries; chloroethylnitrosourea; multi-dimensional data analysis; Biokemi; Biochemistry; Biokemi;

    Abstract : Glutathione transferases (GSTs) are detoxication enzymes involved in the cellular protection against a wide range of reactive substances. The role of GSTs is to catalyze the conjugation of glutathione with electrophilic compounds, which generally results in less toxic products. READ MORE

  6. 4. On bacterial formats in protein library technology

    Author : Per-Åke Löfdahl; Per-Åke Nygren; Mikael Widersten; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Affibody molecule; β-lactamase; combinatorial library; green fluorescent protein GFP ; protein engineering; protein fragment complementation assay PCA ; selection; TEVprotease; TNF-α; Bioengineering; Bioteknik; Enzyme engineering; Enzymteknik;

    Abstract : Millions of years of evolution have resulted in an immense number of different proteins, which participate in virtually every process within cells and thus are of utmost importance for allknown forms of life. In addition, there are several examples of natural proteins which have found use in applications outside their natural environment, such as the use of enzymes infood industry and washing powders or the use of antibodies in diagnostic, bioseparation or therapeutic applications. READ MORE

  7. 5. Microbial Synthetic Biology, Systems Metabolic Engineering and Enzyme Engineering for Advanced Microbial Biodiesel Production with Saccharomyces cerevisiae

    Author : Juan Octavio Valle Rodriguez; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein rational engineering; wax ester synthase; hydrolase; advanced biofuel; fatty acid metabolism; directed evolution; chromosomal delta-integration;

    Abstract : The continuous requirement of transportation biofuels has brought the necessity to establish alternatives permitting low-cost production of biodiesel while being environmentally friendly. Biodiesel production was achieved utilizing Saccharomyces cerevisiae by employing respective enzymes that catalyze the synthesis of fatty acid ethyl esters (FAEEs) based on fatty acyl-CoA molecules and ethanol. READ MORE