Search for dissertations about: "enzyme sequence space"

Showing result 1 - 5 of 7 swedish dissertations containing the words enzyme sequence space.

  2. 1. Experimental and computational exploration of enzyme sequence space

    Author : Elzbieta Rembeza; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; high-throughput-screening; protein annotation; enzyme discovery; enzyme sequence space; oxidases;

    Abstract : Millions of enzymes with desirable features or new exciting activities can be found in organisms occupying diverse niches all around the earth. However, enzyme studies tend to be biased towards characterisation of representatives from eukaryotes, model organisms, or disease-causing bacteria. READ MORE

  4. 2. On bacterial formats in protein library technology

    Author : Per-Åke Löfdahl; Per-Åke Nygren; Mikael Widersten; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Affibody molecule; β-lactamase; combinatorial library; green fluorescent protein GFP ; protein engineering; protein fragment complementation assay PCA ; selection; TEVprotease; TNF-α; Bioengineering; Bioteknik; Enzyme engineering; Enzymteknik;

    Abstract : Millions of years of evolution have resulted in an immense number of different proteins, which participate in virtually every process within cells and thus are of utmost importance for allknown forms of life. In addition, there are several examples of natural proteins which have found use in applications outside their natural environment, such as the use of enzymes infood industry and washing powders or the use of antibodies in diagnostic, bioseparation or therapeutic applications. READ MORE

  5. 3. Chemogenomics: Models of Protein-Ligand Interaction Space

    Author : Helena Strömbergsson; Gerard J Kleywegt; Jarl E S Wikberg; Torgeir R Hvidsten; John Overington; Uppsala universitet; []
    Keywords : ;

    Abstract : The large majority of the currently used drugs are small molecules that interact with proteins. Understanding protein-ligand recognition is thus central to drug discovery and design. Improved experimental techniques have resulted in an immense growth of drug target information. READ MORE

  6. 4. Directed Enzyme Evolution of Theta Class Glutathione Transferase : Studies of Recombinant Libraries and Enhancement of Activity toward the Anticancer Drug 1,3-bis(2-Chloroethyl)-1-nitrosourea

    Author : Anna-Karin Larsson; Bengt Mannervik; Ian Cotgreave; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; glutathione transferase; directed evolution; 1; 3-bis 2-chloroethyl -1-nitrosourea; alkyltransferase; recombinant libraries; chloroethylnitrosourea; multi-dimensional data analysis; Biokemi; Biochemistry; Biokemi;

    Abstract : Glutathione transferases (GSTs) are detoxication enzymes involved in the cellular protection against a wide range of reactive substances. The role of GSTs is to catalyze the conjugation of glutathione with electrophilic compounds, which generally results in less toxic products. READ MORE

  7. 5. Directed Evolution of Glutathione Transferases Guided by Multivariate Data Analysis

    Author : Sanela Kurtovic; Bengt Mannervik; Ulf Hellman; Denis Pompon; Uppsala universitet; []
    Keywords : Biochemistry; DNA shuffling; substrate selectivity; mutant library; glutathione transferase; multivariate data analysis; prodrug; Biokemi;

    Abstract : Evolution of enzymes with novel functional properties has gained much attention in recent years. Naturally evolved enzymes are adapted to work in living cells under physiological conditions, circumstances that are not always available for industrial processes calling for novel and better catalysts. READ MORE