Search for dissertations about: "enzymology"

Showing result 11 - 15 of 74 swedish dissertations containing the word enzymology.

  2. 11. Metal Binding Tags - Characterisation, Use in Bioseparation and Applications of Green Fluorescent Fusion Proteins

    Author : Florent Bernaudat; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; enzymology; E. coli; Immobilised metal ion affinity chromatography; Affinity tags; Green fluorescent protein; Surface plasmon resonance; Genetic engineering; Screening; Proteins; Immunoassay; Biotechnology; Bioteknik;

    Abstract : Most of the recombinant proteins produced nowadays are fused to affinity tags, in order to facilitate their purification through affinity chromato-graphy. Out the different tags available, poly-histidine tags are among the most commonly used and can help purification through immobilised metal ion affinity chromatography (IMAC). READ MORE

  4. 12. Studies of Mesenchymal Progenitor Cells and Tumour Growth, Integrins and Matrix Metalloproteinases

    Author : Lars Bryngelsson Ohlsson; Immunologi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Immunology; Tumour inhibition; cancer; onkologi; cancerology; Cytologi; oncology; Cytology; Biologi; Biology; Medicin människa och djur ; Medicine human and vertebrates ; Integrin matrix metalloproteinase interaction; Immunologi; serologi; transplantation; Proteins; enzymology; Proteiner; enzymologi; serology; Mesenchumal progenitor cells;

    Abstract : In this thesis we characterize the effect of mesenchymal progenitor cells (MPCs) on tumour growth, and show that MPCs can have inhibitory effects on tumour development. We developed a method to subcutaneously transplant tumour cells, cultured in vitro in a pre-formed gelatin matrix, that made it possible to study the early phases of tumour development. READ MORE

  5. 13. Creation of a Nanometer-Scale Toolbox for Molecular Motor Transport-Circuits

    Author : Richard Bunk; Fasta tillståndets fysik; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Coating and surface treatment; materialteknik; Materiallära; Material technology; Produktionsteknik; Production technology; Semiconductory physics; Halvledarfysik; Proteins; enzymology; Proteiner; enzymologi; reumatologi; Physics; Fysik; muskelsystem; Skelett; rheumatology locomotion; muscle system; Skeleton; Biofysik; Biophysics; Biomedicinska vetenskaper; Biomedical sciences; fluorescence microscopy; EBL; T-profile channel; nano-imprinting; cargo; quantum dot; silane; myosin; actin; motor protein; factory-on-a-chip; toolbox; lab-on-a-chip; molecular motor; nanotechnology; Biokemisk teknik; Biotechnology; Bioteknik; Skikt och ytbehandling; Biochemical technology;

    Abstract : This thesis presents studies of molecular motors in interaction with nm-scale structures, as well as the development of a set of tools that can be used for the construction of custom-designed nano-transportation systems. In our studies, we have used the latest nanoscale technology and combined this with advanced results from chemistry and biomedical sciences. READ MORE

  6. 14. Enhanced Enzymatic Hydrolysis of Lignocellulose in Bioethanol Production Substrate Interactions with Surfactants and Polymers

    Author : Johan Börjesson; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; enzymologi; Penicillium brasilianum; SSF; Ethanol; Proteins; enzymology; Proteiner; Protease; Adsorption; PEG; Surfactant; Hydrolysis; Lignocellulose; Cellulase; Hypocrea jecorina; Trichoderma reesei; Cellulose;

    Abstract : Ethanol produced from cellulose is a promising future alternative fuel. The production process has three main steps: (i) pretreatment of raw material to increase degradability; (ii) enzymatic hydrolysis of cellulose to produce glucose; (iii) fermentation of glucose into bioethanol. READ MORE

  7. 15. Structural studies of drug targets and a drug metabolizing enzyme

    Author : Daniela Cederfelt; U. Helena Danielson; Doreen Dobritzsch; Inari Kursula; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biophysics; Protein structure; X-ray crystallography; Cryogenic electron microscopy; Enzymology; Drug discovery; Pyrimidine degradation; Biokemi; Biochemistry;

    Abstract : The work presented in this thesis describes how structural information about a protein can be acquired, and how it can be used to answer scientific questions about proteins’ function, their dynamic behaviour and their interactions with other proteins or ligands.The catalytic function of the pyrimidine-degrading, drug metabolizing enzyme β-ureidopropionase (βUP) is dependent on the shift between oligomeric states. READ MORE