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Showing result 1 - 5 of 32 swedish dissertations matching the above criteria.

  2. 1. Array-based Autoantibody Profiling and Epitope Mapping

    Author : Arash Zandian; Peter Nilsson; Jochen M. Schwenk; Maja Neiman; Morten Nielsen; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody; antigens; peptide; epitope mapping; autoimmunity; autoantibodies; microarrays; Bioteknologi; Biotechnology;

    Abstract : Antibodies are a class of proteins that are made by the immune system to recognize harmful organisms and molecules. Their exceptional capability of specifically recognizing molecules has been investigated for over a century and information thereof has been utilized for a variety of applications including vaccine and generation of therapeutic antibodies. READ MORE

  4. 2. Epitope mapping of antibodies towards human protein targets

    Author : Barbara Hjelm; Mathias Uhlén; Larry Gold; KTH; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; antibody; antibody validation; biomarker; epitope mapping; peptide array; proteomics; RBM3; staphylococcal surface display;

    Abstract : This thesis, based on five research papers, presents results from development and evaluation ofmethods for identifying the interaction site of antibodies on their antigens and the functional investigation of these in different assays. As antibodies have proven to be invaluable tools in diagnostics, therapy and basic research, the demand of characterizing these binding molecules has increased. READ MORE

  5. 3. Utilizing Solid Phase Cloning, Surface Display And Epitope Information for Antibody Generation and Characterization

    Author : Francis Jingxin Hu; Johan Rockberg; John McCafferty; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody engineering; antibody affinity maturation; combinatorial protein engineering; epitope mapping; FACS; HER2; complement C5; Staphylococcal surface display; surface display.; Bioteknologi; Biotechnology;

    Abstract : Antibodies have become indispensable tools in diagnostics, research and as therapeutics. There are several strategies to generate monoclonal antibodies (mAbs) in order to avoid the drawbacks of polyclonal antibodies (pAbs) for therapeutic use. READ MORE

  6. 4. Characterization of antibody specificity using peptide array technologies

    Author : Björn Forsström; Mathias Uhlén; Markus Templin; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Antibody; Epitope mapping; Peptide array; Suspension bead array; Antigen; Specificity; Cross-reactivity; Immunization; Immunogenicity; Bioteknologi; Biotechnology;

    Abstract : Antibodies play an important role in the natural immune response to invading pathogens. The strong and specific binding to their antigens also make them indispensable tools for research, diagnostics and therapy. READ MORE

  7. 5. Cell line and protein engineering tools for production and characterization of biologics

    Author : Anna-Luisa Volk; Johan Rockberg; Mathias Uhlen; Dickson Alan; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Pharmaceutical proteins; precision medicine; antibody engineering; epitope mapping; cell line development; split-GFP; Bioteknologi; Biotechnology;

    Abstract : Our increasing understanding of disease mechanisms coupled with technological advances has facilitated the generation of pharmaceutical proteins, which are able to address yet unmet medical needs. Diseases that were fatal in the past can now be treated with novel biological medications improving and prolonging life for many patients. READ MORE