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Showing result 1 - 5 of 9 swedish dissertations matching the above criteria.

  2. 1. A study of some operational parameters in expanded bed adsorption of proteins

    Author : Rolf Hjort; Uppsala universitet; []
    Keywords : ;

    Abstract : .... READ MORE

  4. 2. Fast isolation of proteins from bioreactors using affinity chromatography techniques

    Author : Eva Pålsson; Avdelningen för klinisk genetik; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; chromatography; protein purification; analyses; dispersion; chromatography matrices; Biochemistry; Biokemi; Metabolism; expanded bed; metabolism; Biotechnology; Bioteknik;

    Abstract : The purification of proteins from raw materials like fermentation broth and other biological sources usually involves several purification steps and is rather time consuming and expensive. This is a major concern in the production of many important protein products e.g. pharmaceuticals such as insulin and blood coagulation factors. READ MORE

  5. 3. Production and engineering of a xyloglucan endo-transglycosylase from Populus tremula x tremuloides

    Author : Maria Henriksson; Tuula Teeri; KTH; []
    Keywords : LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; xyloglucan endo-transglycosylase; retaining glycoside hydrolase; glycosynthase; Pichia pastoris; fed-batch fermentation; expanded-bed adsorption; Plant biotechnology; Växtbioteknik;

    Abstract : The aim of this work was to develop a production process for the enzyme xyloglucan endo-transglycosylase from Populus tremula x tremuloides (PttXET16-34). The natural transglycosylating activity of this enzyme has previously been employed in a XET-Technology. READ MORE

  6. 4. Polyelectrolytes and Polycomplexes in Biotechnology

    Author : Maria Dainiak; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; expanded bed adsorption.; affinity precipitation; Fab fragments; monoclonal antibodies; glyceraldehyde-3-phosphate dehydrogenase; misfolded protein; chaperone; poly N-ethyl-4-vinylpyridinium bromide ; poly methacrylic acid ; poly acrylic acid ; Key words: polyelectrolyte; polyelectrolyte complexes; Biotechnology; Bioteknik; Chemical technology and engineering; Kemiteknik och kemisk teknologi;

    Abstract : Polyelectrolytes and polyelectrolyte complexes (PECs) are characterized by many useful properties that endow them with high potential in different areas of biotechnology. This thesis describes the development and analyses of some new systems and methods utilizing poly(acrylic acid) (PAA), poly(methacrylic acid) (PMA), poly (N-ethyl-4-vinylpyridinium bromide) (PEVP) and PMA-PEVP complexes. READ MORE

  7. 5. Effects of fusion tags on protein partitioning In aqueous two-phase systems and use in primary protein recovery

    Author : Cynthia Hassinen; KTH; []
    Keywords : E. coli; Aqueous two-phase systems; Fusion proteins; Hydrophobic interaction chromatography; Expanded bed adsorption; Beta-galactosidase; Klenow polymerase; Z-domain; Peptide tags;

    Abstract : The two techniques aqueoustwo-phase partitioning and expanded bed adsorption that bothare suitable for primary protein recovery were studied. Most ofthe work was focused on partition in aqueous two-phase systemsand in particular on the possibility to effect the partitionbehaviour by fusion of short peptide tags or protein domains tothe target protein. READ MORE