Search for dissertations about: "expanded-bed adsorption"

Showing result 6 - 9 of 9 swedish dissertations containing the words expanded-bed adsorption.

2. 6. Primary Recovery of Biologically Active Compounds Using Macroporous Monolithic Hydrogels

   Author : Amro Hanora; Bioteknik; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Downstream processing; macroporous; Biologically active compounds; Biotechnology; Läkemedelsteknik och relaterad teknik; Pharmaceutical and related technologies; Polymerteknik; biopolymers; Biokemisk teknik; Polymer technology; Makromolekylär kemi; Biochemical technology; Affinity chromatography; Ion exchange chromatography; Expanded bed adsorption chromatography.; Macromolecular chemistry; High through put screening; lactica acids; Endotoxin; Plasmid DNA; Cryogel columns; Bioteknik;

   Abstract : The completion of sequencing of human and other mammalian genomes opens a new era for drug development. New approaches have been proposed for tackling diseases such as development of small molecule therapies based on understanding of human genome and proteome, new proteinous based therapies, where the therapeutic protein can inhibit or modify a certain metabolic pathway or DNA-based therapies delivering a gene into the cell (e. READ MORE

4. 7. Strategies for facilitated protein recovery after recombinant production in Escherichia coli

   Author : My Hedhammar; Sophia Hober; Alois Jungbauer; KTH; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ion-exchange chromatography; protein A; Z; Zbasic; Zacid; fusion protein; proteolytic cleavage; immobilised protease; flow cytometry; inclusion bodies; solid-phase refolding; Molecular biology; Molekylärbiologi;

   Abstract : The successful genomic era has resulted in a great demand for efficient production and purification of proteins. The main objective of the work described in this thesis was to develop methods to facilitate recovery of target proteins after recombinant production in Escherichia coli. READ MORE

5. 8. Influence of Escherichia coli feedstock properties on the performance of primary protein purification

   Author : Mattias Råvik; Andres Veide; Åsa Emmer; KTH; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Downstream processing; cell surface properties; surface plasmon resonance; contact angle measurements; microelectrophoresis; aqueous two-phase partitioning; filtration; EBA adsorbents; high-pressure homogenisation; Biochemistry; Biokemi;

   Abstract : Abstract The aim of the present study was to increase the understanding of how the cell surface properties affect the performance of unit operations used in primary protein purification. In particular, the purpose was to develop, set up and apply methods for studies of cell surface properties and cell interactions. READ MORE

6. 9. Design, selection and production of recombinant proteins for prevention of infectious diseases

   Author : Marianne Hansson; KTH; []
   Keywords : ;

   Abstract : The development of subunit vaccines is presently one of themain strategies for the generation of new vaccines againstinfectious diseases. The use of recombinant DNA techniques hasfacilitated the develoment of new strategies for constructionand production of subunit vaccines. READ MORE