Search for dissertations about: "fed-batch control"

Showing result 6 - 10 of 32 swedish dissertations containing the words fed-batch control.

  2. 6. The temperature-limited fed-batch technique for control of Escherichia coli cultures

    Author : Marie Svensson; Sven-Olof Enfors; John Villadsen; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Endotoxin; Escherichia coli; fed-batch technique; glucose-limitation; mathematical model; Bioengineering; Bioteknik;

    Abstract : The objective of this study was to investigate the physiology and productivity in Escherichia colicultures with emphasis on the temperature-limited fed-batch (TLFB) culture. The TLFB techniquecontrols the oxygen consumption rate of the growing culture by a gradually declining temperaturefrom 37-35 °C down to 20-18 °C. READ MORE

  4. 7. Large-scale Bioreactor Phenomena and Characteristics; Effects on E.coli fed batch fermentations on 30m3 scale

    Author : Gustav Silfversparre; KTH; []
    Keywords : Large-scale; E.coli; fed batch; concentration gradients; mixing;

    Abstract : Due to economical and practical limitations the fluiddynamics and mass transport properties are less optimal inlarge-scale bioreactors compared to lab-scale bioreactors. Incombination with the microbial reaction rates this creates amuch more dynamic environment for cells cultivated inlarge-scale bioreactors compared to lab-scale bioreactors. READ MORE

  5. 8. Ethanol from Lignocellulose: Physiological Effects of Inhibitors and Fermentation Strategies

    Author : Mohammad Taherzadeh Esfahani; Chalmers tekniska högskola; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; fermentation; dilute-acid hydrolyzate; ethanol; hydroxymethyl furfural; furfural; acetic acid; lignocellulose; fed-batch; yeast; pantothenate; control; inhibition; HMF;

    Abstract : Fermentative ethanol production from dilute-acid hydrolyzates of wood using the yeast Saccharomyces cerevisiae was investigated. Of known inhibitors in hydrolyzates, acetic acid, furfural and hydroxymethyl furfural (HMF) were found in the highest concentrations (up to about 10 g/l). READ MORE

  6. 9. Improving Ethanol Production from Lignocellulose Hydrolyzates: Fed-batch Fermentation, Yeast Cultivation and Strain Development for Increased Tolerance

    Author : Anneli Petersson; Avdelningen för kemiteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Kemiteknik och kemisk teknologi; Chemical technology and engineering; Saccharomyces cerevisiae; fed-batch control; Ethanol production; dilute acid hydrolyzate;

    Abstract : Ethanol production by the yeast Saccharomyces cerevisiae from dilute acid lignocellulose hydrolyzate was studied with the aim to improve fuel ethanol production. Specifically, the problem of inhibition was addressed. READ MORE

  7. 10. Growth rate control of periplasmic product retention in Escherichia coli

    Author : Emma Bäcklund; Gen Larsson; Stefan Schmidt; Amelie Eriksson Karlström; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Escherichia coli; fed-batch; outer membrane proteins; recombinant proteins; specific growth rate; periplasmic retention; phosphotransferase system; high cell density cultivation; acetate formation; Bioengineering; Bioteknik;

    Abstract : The recombinant product is secreted to the periplasm in many processes where E. coli is used as host. One drawback with secretion is the undesired leakage of the periplasmic products to the medium. The aim of this work was to find strategies to influence the periplasmic retention of recombinant products. READ MORE