Search for dissertations about: "fed-batch technique"

Showing result 1 - 5 of 11 swedish dissertations containing the words fed-batch technique.

2. 1. The temperature-limited fed-batch technique for control of Escherichia coli cultures

   Author : Marie Svensson; Sven-Olof Enfors; John Villadsen; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Endotoxin; Escherichia coli; fed-batch technique; glucose-limitation; mathematical model; Bioengineering; Bioteknik;

   Abstract : The objective of this study was to investigate the physiology and productivity in Escherichia colicultures with emphasis on the temperature-limited fed-batch (TLFB) culture. The TLFB techniquecontrols the oxygen consumption rate of the growing culture by a gradually declining temperaturefrom 37-35 °C down to 20-18 °C. READ MORE

4. 2. Probing Control : Analysis and Design with Application to Fed-Batch Bioreactors

   Author : Stéphane Velut; Institutionen för reglerteknik; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Elektronik och elektroteknik; Electronics and Electrical technology; Large scale fermentations; Extremum control; Feeding strategy; Probing control; Fed-batch bioreactors; Automation; robotics; control engineering; Automatiska system; robotteknik; reglerteknik;

   Abstract : In most control problems the objective is to control the output at a desired value in spite of disturbances. In some cases, the best setpoint is not known a priori and it should be found online to optimize the process performance. This thesis examines a probing strategy that can be applied for this class of problems. READ MORE

5. 3. Impact of glucose uptake rate on recombinant protein production in Escherichia coli

   Author : Emma Bäcklund; Gen Larsson; Olle Holst; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; AIDA-autotransporter; Escherichia coli; fed-batch; glucose uptake rate; integral membrane proteins; outer membrane proteins; periplasmic retention; phosphotransferase system; recombinant proteins; specific growth rate; surface expression; Biology; Biologi;

   Abstract : Escherichia coli (E. coli) is an attractive host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve high product quantities. READ MORE

6. 4. Modelling and control of microbial fed-batch and pH-auxostat processes

   Author : Thi Bich Hop Pham; KTH; []
   Keywords : ;

   Abstract : Glucose and energy metabolism ofSaccharomyces cerevisiaehas been studied for decades,yet the exact location of the so called "bottleneck" thatcauses overflow metabolism resulting in aerobic ethanolformation remains unknown. However, this does not completelyhamper the modelling, simulation and development of methods tocontrol the aerobic ethanol formation under processconditions. READ MORE

7. 5. Feeding Strategies Based on Probing Control for E. coli and V. cholerae Cultivations

   Author : Lena de Maré; Institutionen för reglerteknik; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; robotics; control engineering; Automatiska system; robotteknik; reglerteknik; Automation; Temperature; Mid-ranging control; Amino acid; Complex media; Fed-batch bioreactors; Probing control; Biotechnology; Bioteknik; Parameter estimation; Feeding strategies;

   Abstract : The recombinant DNA techniques have made it possible to produce many different proteins for a wide range of applications. The vector encoding for the recombinant protein is often inserted in the bacterium E. coli as it is a well studied and a well-known organism. To achieve a high productivity, it is important to reach a high cell density. READ MORE