Search for dissertations about: "flow immunoassay"

Showing result 1 - 5 of 23 swedish dissertations containing the words flow immunoassay.

2. 1. Sampling, sample treatment, assay and real time monitoring of biotechnological processes using an integrated flow-injection system

   Author : Mohammad Mazlomi; Bioteknik; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Bioprocess; Sampling; Sample treatment; On-line monitoring; Real time monitoring; Flow injection; Immunoassay; Fermentation;

   Abstract : Microorganisms can be used for production of pharmaceutical substances. With the introduction of gene technology, it is possible to transfer information from one organism to the other, thereby making the receiving organism capable of producing substances that it did not so before. This development is very important for e.g. READ MORE

4. 2. Enzyme Immunoassay in Combination with Liquid Chromatography for Sensitive and Selective Determination of Drugs in Biosamples

   Author : Ulf Lövgren; Centrum för analys och syntes; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; amplification; alkaline phosphatase; enzyme immunoassay; liquid chromatography; flow-injection; micellar; Analytisk kemi;

   Abstract : Two different sensitive microtitre-plate based enzyme immunoassays were developed and evaluated for determination of the model drug compound, budesonide, in blood plasma. In the first approach, high sensitivity was obtained by using amperometric detection in a flow injection system for determination of the enzyme product p-aminophenol. READ MORE

5. 3. Membrane-Assisted Isoform ImmunoAssay : Separation and determination of protein isoforms

   Author : Maria Lönnberg; Carl G M Magnusson; Uppsala universitet; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Bioteknik; Bioengineering; Bioteknik; Surface Biotechnology; ytbioteknik;

   Abstract : Proteins exist in a variety of isoforms with minor differences, mostly due to their glycosylation patterns, which can modulate their biological functions. It seems to be of clinical relevance to measure the isoform-distribution.Thesis describes a novel technology named Membrane-Assisted Isoform ImmunoAssay (MAIIA). READ MORE

6. 4. Development and Application of Enzyme- and Antibody-based Assays with Emphasis on Selectivity, Sensitivity and Stability

   Author : Catalin Nistor; Centrum för analys och syntes; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; stability; sensitivity; selectivity; cross-reactivity; sample matrix effects; environmental pollutants; enzyme biosensor; immunoassay; Analytical chemistry; Analytisk kemi;

   Abstract : This thesis presents the development, optimisation, and various applications of enzyme-based biosensors and immunoassays, mainly with relevance to environmental analysis. the thesis discusses problems and possible solutions associated with selectivity, sensitivity and robustness of such bioassays with emphasis on various immunoasssay configurations for determination of surfactants, phenols, or drugs, and amperometric biosensors based on enzymes (e. READ MORE

7. 5. Sample Handling Techniques in Biological and Environmental Applications Emphasizing Biomolecular Recognition and MALDI-TOF MS

   Author : Patrik Önnerfjord; Reumatologi och molekylär skelettbiologi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; protein identification; microchip IMER; microdispensing; MALDI-TOF MS; fluorescence polarization; flow immunoassay; pesticide immunoassays; phenol biosensor; restricted access media; SPE; Analytical chemistry; Analytisk kemi;

   Abstract : The use of restricted access media and other stationary phases for clean-up and trace enrichment of environmental samples coupled on-line to liquid chromatography using diode array detection as well as thermospray MS is described. This on-line configuration was compared with commercially available ELISAs for the determination of triazines in complex matrices, showing superior precision and accuracy with the chromatographic method. READ MORE