Search for dissertations about: "fluorescence anisotropy"

Showing result 1 - 5 of 32 swedish dissertations containing the words fluorescence anisotropy.

  2. 1. Two-Photon Excited Fluorescence Depolarisation : Experimental and Theoretical Development

    Author : Linus Ryderfors; Emad Mukhtar; Lennart B.-Å. Johansson; Yehudi K. Levine; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Two-photon excitation; Fluorescence; Fluorescence depolarisation; Fluorescence anisotropy; Time-correlated single photon counting; Gold nanoparticles; Rotational diffusion; Excited state symmetry; Extended Förster theory; Orientation correlation functions; Donor-donor energy migration DDEM ; Chemical physics; Kemisk fysik;

    Abstract : We have studied fundamental aspects of time-resolved two-photon excited fluorescence depolarisation. The thesis presents experimental as well as theoretical progress. READ MORE

  4. 2. Fluorescence studies of complex systems : organisation of biomolecules

    Author : Denys Marushchak; Lennart B.A. Johansson; Alexander Lyubartsev; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Fluorescence anisotropy; BODIPY; homo and hetero dimerisation; protein aggregates; protein polymer structures; actin polymerisation; FRET; donor–acceptor energy transfer DAET; donor-donor energy migration DDEM; homotransfer; Monte Carlo simulation; MC; Brownian dynamics; BD; Genetic Algorithm; GA.; Ganglioside GM1; Biophysical chemistry; Biofysikalisk kemi;

    Abstract : The homo and hetero dimerisation of two spectroscopically different chromophores were studied, namely: 4,4-difluoro-4-bora-3a,4a-diazas-indacene (g-BODIPY) and its 5-styryl-derivative (r-BODIPY). Various spectroscopic properties of the r-BODIPY in different common solvents were determined. READ MORE

  5. 3. Dynamics of Enzyme Immobilization in Mesoporous Silica Particles

    Author : Pegah Sadat Nabavi Zadeh; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; fluorescence spectroscopy; real-time monitoring.; fluorescence anisotropy; pore filling; Enzyme immobilization; mesoporous silica; kinetics; rotational mobility; rate of immobilization;

    Abstract : The focus of this thesis is placed on the dynamic behavior of proteins in confining environments which is studied as a scientific challenge for a deeper understanding of the immobilization mechanism and a better designing of enzyme immobilization in porous materials. Enzymes are immobilized in porous materials to improve the enzyme activity and simplify their purification from the product solution in biocatalytic applications. READ MORE

  6. 4. Optimizing Enzyme Immobilization in Mesoporous Silica - A Spectroscopic Study of the Dynamics and Spatial Distribution of the Confined Enzymes

    Author : Pegah Sadat Nabavi Zadeh; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; fluorescence spectroscopy; dynamics; mesoporous silica; Immunogold staining; Transmission electron microscopy; anisotropy; microviscosity; real-time monitoring; Cy5.; Förster resonance energy transfer; Enzyme immobilization; Cy3;

    Abstract : Enzymes as biological catalysts are immobilized in porous materials to improve the enzyme activity and simplify their purification from the reaction media in biocatalytic applications. Mesoporous silica (MPS) particles are used as a promising support material for the immobilization of enzymes. READ MORE

  7. 5. Light polarization in single molecule spectroscopy: From visualization of structural anisotropy and energy transfer to super-resolution imaging with orientational contrast

    Author : Daniel Thomsson; Kemisk fysik; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Single-molecule spectroscopy; π-conjugated polymers; blinking; fluorescence brightness; low temperature; polarization anisotropy; 2D polarization; energy transfer; super resolution; Fourier transform;

    Abstract : Π-conjugated polymers are an important class of materials which can be used in e.g. photoelectric (solar cells) or electroluminescent (displays) devices. These materials are implemented in a number of applications these days since a lot is already known about them. READ MORE