Search for dissertations about: "fluorescence brightness"

Showing result 1 - 5 of 11 swedish dissertations containing the words fluorescence brightness.

  2. 1. Laboratory x-ray fluorescence tomography

    Author : Jakob C. Larsson; Hans Hertz; Anna Burvall; Ulrich Vogt; Norbert Pelc; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; x-ray; fluorescence; x-ray fluorescence; nanoparticle; xrf; xfct; tomography; Fysik; Physics;

    Abstract : X-ray fluorescence (XRF) tomography is an emerging bio-imaging modality with potential for high-resolution molecular imaging in 3D. In this technique the fluorescence signal from targeted nanoparticles (NPs) is measured, providing information about the spatial distribution and concentration of the NPs inside the object. READ MORE

  4. 2. Transient State Fluorescence Microscopy - method development and biological applications : Exploiting the dark states of fluorophores to measure oxygen concentrations, redox state, Förster resonance energy transfer and membrane viscosity

    Author : Thiemo Spielmann; Jerker Widengren; Karsten König; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Transient States imaging TRAST ; Triplet State imaging; fluorescence microscopy; modulated excitation; triplet state; radical state; trans-cis isomerisation;

    Abstract : Due to their long lifetime, triplet, redox and other transient states of fluorophores are highly sensitive to the micro-environment. Imaging their spatial distribution in biological samples can therefore help answer interesting questions about the metabolism, molecular interactions and dynamics in living cells. READ MORE

  5. 3. Light polarization in single molecule spectroscopy: From visualization of structural anisotropy and energy transfer to super-resolution imaging with orientational contrast

    Author : Daniel Thomsson; Kemisk fysik; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Single-molecule spectroscopy; π-conjugated polymers; blinking; fluorescence brightness; low temperature; polarization anisotropy; 2D polarization; energy transfer; super resolution; Fourier transform;

    Abstract : Π-conjugated polymers are an important class of materials which can be used in e.g. photoelectric (solar cells) or electroluminescent (displays) devices. These materials are implemented in a number of applications these days since a lot is already known about them. READ MORE

  6. 4. Concentration measurements in single particle microscopy

    Author : Magnus Röding; Göteborgs universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; diffusion; fluorescence microscopy; nanoparticle characterization; number concentration; optical wide-field microscopy; single particle tracking; Smoluchowski process; diffusion;

    Abstract : The topic of this thesis is the introduction of two novel methods for using single particle microscopy as a tool for absolute number concentration measurements of Brownian particles. The key idea of both methods is that in order to estimate number concentration, the size of the (three-dimensional) particle detection region has to be estimated. READ MORE

  7. 5. Fluorescence-based methods to probe long-lived transient states for biomolecular studies

    Author : Heike Hevekerl; Jerker Widengren; Martin Hof; KTH; []
    Keywords : Fysik; Physics;

    Abstract : Fluorescence spectroscopy and imaging are wide-spread tools in life science. Themain read-out parameters are still fluorescence intensity and wavelength, but given thebenefits of multi-parameter characterization there are also good reasons to consideradditional fluorescence-based read-out parameters. READ MORE