Search for dissertations about: "fluorescence protein"

Showing result 1 - 5 of 356 swedish dissertations containing the words fluorescence protein.

  2. 1. Perplexing Protein Puzzles

    Author : Stina Lindman; Biofysikalisk kemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; calbindin D9k; split-GFP; protein G B1; pKa-values; protein reconstitution; Electrostatic interactions; protein stability; protein denaturation; heteronuclear NMR; pH titrations;

    Abstract : Protein structure and stability are inherent in the amino acid sequence and governed by non-covalent interactions. The cooperation between forces is, however, perplexing and not well understood. In order to elucidate and predict protein folding and stability, detailed studies of non-covalent interactions are required. READ MORE

  4. 2. Advanced Fluorescence Microscopy to Study Plasma Membrane Protein Dynamics

    Author : Joachim Piguet; Horst Vogel; Ruud Hovius; Jerker Widengren; Antoine Triller; Switzerland Ecole Polytechnique Fédérale de Lausanne (EPFL); []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; fluorescence; microscopy; plasma membrane; membrane proteins; membrane proteins diffusion; single-molecule imaging; single-molecule tracking; pentameric ligand-gated ion channels; nicotinic acetylcholine receptor; serotonin receptor; synapse; neuromuscular junction; post-synaptic scaffold; rapsyn; myopathies; fluorescent proteins; Förster resonance energy transfer; FRET imaging; protein-protein interactions; protein trafficking; photo-activatable proteins; Biological Physics; Biologisk fysik;

    Abstract : Membrane protein dynamics is of great importance for living organisms. The precise localization of proteins composing a synapse on the membrane facing a nerve terminus is essential for proper functioning of the nervous system. In muscle fibers, the nicotinic acetylcholine is densely packed under the motor nerve termini. READ MORE

  5. 3. Protein stabilization. Some methods and mechanisms

    Author : Maria Andersson; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; sorbitol; protein stability; preferential exclusion; polyol; poly-NIPAAm; polymer; poly ethyleneimine ; polyelectrolyte; oxidation; NMR spectroscopy; lysozyme; lactate dehydrogenase; fluorescence; dynamic light scattering; complex; additive; circular dichroism; stabilization; trypsin; thermo-reversible polymer.; Biotechnology; Bioteknik;

    Abstract : The effect of different additives on protein stability was studied, and the basis for stabilization was investigated at molecular level. The addition of sorbitol enhanced thermal stability of hen egg white lysozyme in aqueous solution. READ MORE

  6. 4. Fluorescence studies of complex systems : organisation of biomolecules

    Author : Denys Marushchak; Lennart B.A. Johansson; Alexander Lyubartsev; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Fluorescence anisotropy; BODIPY; homo and hetero dimerisation; protein aggregates; protein polymer structures; actin polymerisation; FRET; donor–acceptor energy transfer DAET; donor-donor energy migration DDEM; homotransfer; Monte Carlo simulation; MC; Brownian dynamics; BD; Genetic Algorithm; GA.; Ganglioside GM1; Biophysical chemistry; Biofysikalisk kemi;

    Abstract : The homo and hetero dimerisation of two spectroscopically different chromophores were studied, namely: 4,4-difluoro-4-bora-3a,4a-diazas-indacene (g-BODIPY) and its 5-styryl-derivative (r-BODIPY). Various spectroscopic properties of the r-BODIPY in different common solvents were determined. READ MORE

  7. 5. Nanofluidics for Static and Dynamic DNA-Protein Interaction Studies - Repair of Double-Strand Breaks from a Single-Molecule Perspective

    Author : Robin Öz; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Single-molecule; Protein; NHEJ; Dynamic; DNA repair; HR; Biomolecule interactions; Microscopy; Fluorescence; Nanofluidics;

    Abstract : Double-strand breaks (DSBs) is one of the most lethal forms of DNA damage. A single DSB may result in stalling of vital cellular machineries, and thus, requires immediate measures by the cell. READ MORE