Search for dissertations about: "galactosidase"

Showing result 1 - 5 of 40 swedish dissertations containing the word galactosidase.

  2. 1. Lipid sponge phase nanostructures as carriers for enzymes

    Author : Maria Valldeperas Badell; NanoLund: Centre for Nanoscience; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Sponge phase; lipid liquid crystals; DGMO; GMO-50; polysorbate 80; encapsulation of proteins; Aspartic protease; β-galactosidase; X-ray and neutron scattering; Raman spectroscopy; adsorption; cryo-TEM;

    Abstract : Nonlamellar lipid liquid crystalline phases have many potential applications, such as for drug delivery, protein encapsulation or crystallization. Lipid liquid crystalline sponge phase (L3) has so far not been very much considered in these applications, in spite of apparent advantages in terms of its flexibility and capacity of forming large aqueous pores able to encapsulate large bioactive molecules. READ MORE

  4. 2. Galactoglucomannan-degrading enzymes from Aspergillus niger

    Author : Pia Ademark; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; beta-mannanase; Aspergillus niger; beta-mannosidase; alpha-galactosidase; purification; hydrolysis; cloning; galactoglucomannan; expression; Biochemistry; Metabolism; Biokemi;

    Abstract : Galactoglucomannan and galactomannan are among the most abundant plant polysaccharides in nature. The central enzymes involved in their degradation, i.e., endo-1,4-beta-mannanase (beta-mannanase, EC 3. READ MORE

  5. 3. Effects of fusion tags on protein partitioning In aqueous two-phase systems and use in primary protein recovery

    Author : Cynthia Hassinen; KTH; []
    Keywords : E. coli; Aqueous two-phase systems; Fusion proteins; Hydrophobic interaction chromatography; Expanded bed adsorption; Beta-galactosidase; Klenow polymerase; Z-domain; Peptide tags;

    Abstract : The two techniques aqueoustwo-phase partitioning and expanded bed adsorption that bothare suitable for primary protein recovery were studied. Most ofthe work was focused on partition in aqueous two-phase systemsand in particular on the possibility to effect the partitionbehaviour by fusion of short peptide tags or protein domains tothe target protein. READ MORE

  6. 4. Gene fusion in protein engineering : design of novel peptides and bifunctional enzymes

    Author : Sarah Fredriksson; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Cadmium; Bifunctional enzyme; Catalytic triad; Channeling; Coenzyme recycling; Galactose dehydrogenase; ß-Galactosidase; Gene fusion; Hexapeptide; Lactate dehydrogenase; Linker region; Multifunctional enzymes; Oligomeric protein; NADH; Phage; OmpA; Biochemistry; Biokemi; metabolism; Biotechnology; Bioteknik;

    Abstract : The gene fusion technique is widely used in protein engineering and in this thesis it constitutes the basis of both the construction of artificial bifunctional enzymes and the display of two novel peptides. Artificial bifunctional enzymes have been used as model systems in the studies of native multifunctional enzymes and proximity effects within these complexes. READ MORE

  7. 5. Enzymatic conversion of β-mannans: Analysing, evaluating and modifying transglycosylation properties of glycoside hydrolases

    Author : Mathias Wiemann; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; beta-mannanase; alpha-galactosidase; enzyme engineering; transglycosylation; MALDI-TOF MS; HPLC;

    Abstract : Retaining glycoside hydrolases are enzymes that catalyse the breakdown down of glycans through hydrolysis. Due to the double-replacement mechanism of the retaining glycoside hydrolases (GHs), which form an intermediate with part of the glycan covalently attached to the enzyme, some GHs are able to catalyse synthesis reactions called transglycosylation. READ MORE