Search for dissertations about: "gel-electrophoresis"

Showing result 1 - 5 of 206 swedish dissertations containing the word gel-electrophoresis.

  1. 1. Concatemerization of Synthetic Oligonucleotides, Assembly on Surface and Ligand Interactions

    Author : Lu Sun; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; DNA formation; DNA concatemer; self-assemble; gel electrophoresis; DNA-binding; hybridization; QCM-D;

    Abstract : DNA nanotechnology has become an important research field because of its advantages in high predictability and accuracy of base-paring recognition. DNA concatemer, one of the simplest DNA constructs in shape, have been found as efficient signal amplifiers in several biosensors for different applications. READ MORE

  2. 2. Formation and Characterization of DNA Concatemers for Single Molecule Mechanism Studies

    Author : Lu Sun; Chalmers University of Technology; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; concatemers; hybridization; DNA; gel electrophoresis; self-assembly; constructs; nanotechnology;

    Abstract : Well known as genetic information carrier, DNA however, in the past three decade experienced a revolution to become a fashionable fabrication material in nanotechnology as a consequence of the high predictability and accuracy of base-paring recognition. Although DNA can be constructed in many fancy 2D and 3D structures, this thesis work focused on constructing one-dimension linear DNA concatemers in order to study sequence specific DNA-ligand binding at the single-molecule level. READ MORE

  3. 3. Concatemerization of Synthetic Oligonucleotides

    Author : Lu Sun; Chalmers University of Technology; []
    Keywords : NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; NATURAL SCIENCES; gel electrophoresis; DNA-binding; QCM-D; DNA concatemer; hybridization; DNA formation; self-assembly;

    Abstract : DNA nanotechnology has become an important research field because of its advantages in high predictability and accuracy of base-paring recognition. For example, the DNA concatemer, one of the simplest DNA constructs in shape, has been used to enhance signals in biosensing. READ MORE

  4. 4. In vitro and in vivo studies of salivary films at solid/liquid interfaces

    Author : Ida Svendsen; Malmö högskola; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; saliva; proteins; adsorption; ellipsometry; gel electrophoresis;

    Abstract : A conditioning film, the pellicle, of which many salivary proteins areimportant constituents, covers the surfaces present in the mouth. The pellicleforms in a selective adsorption process, and it has protective and lubricatingfunctions as well as an influence on the adherence of oral microbes thatultimately leads to the development of dental plaque. READ MORE

  5. 5. Groove-binding Unsymmetrical Cyanine Dyes for DNA Applications

    Author : Maja Eriksson; Chalmers University of Technology; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; polarized spectroscopy; bacteriophage T5; cryo-transmission electron microscopy; dynamic light scattering; DNA; intercalation; cyanine dyes; groove-binding; fluorescence spectroscopy; gel electrophoresis;

    Abstract : This work focuses on interactions between DNA and a group of newly developed unsymmetrical cyanine dyes. The aim of the work was to discover fluorescent dyes that can be used in biophysical studies of natural DNA where minimum perturbation of the DNA-helix is essential, where a slow dissociation rate of the dyes is needed and where the ionic strength is an important parameter. READ MORE