Search for dissertations about: "gene expression and Escherichia coli"

Showing result 1 - 5 of 93 swedish dissertations containing the words gene expression and Escherichia coli.

2. 1. Into the Membrane and Beyond : Improving Membrane Protein Over-Expression in Escherichia coli

   Author : Mirjam Klepsch; Jan-Willem de Gier; Reinhard Grisshammer; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; membrane protein over-expression; proteomics; T7 RNA polymerase; peptide transport; strain engineering; Biochemistry; Biokemi; Biophysical chemistry; Biofysikalisk kemi; biokemi; Biochemistry;

   Abstract : Membrane proteins fulfil a wide variety of essential functions in the cell and many are (potential) drug targets. Since their natural abundance is usually very low, most membrane proteins have to be over-expressed for functional and structural studies. READ MORE

4. 2. Studies on translation initiation and gene expression in Escherichia coli

   Author : Ernesto I. Gonzalez de Valdivia; Leif Isaksson; Stephen Douthwaite; Stockholms universitet; []
   Keywords : Downstream region; NGG codons; abortive translation; drop-off; Shine-Dalgarno sequence; gene expression and Escherichia coli; NATURAL SCIENCES; NATURVETENSKAP;

   Abstract : In prokaryotes, several mRNA sequences surrounding the initiation codon have been found to influence the translation process; these include the downstream region and its codon context, the Shine-Dalgarno sequence and the S1 ribosomal protein-binding site. In this thesis, the purpose has been to study the role of the downstream region and Shine-Dalgarno-like sequences on early translation elongation and gene expression in Escherichia coli. READ MORE

5. 3. Protein production and purification in structural genomics

   Author : Martin Hammarström; Per-Åke Nygren; Torleif Härd; Helena Berglund; Herman van Tilbeurgh; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Recombinational cloning; Escherichia coli; gene expression; fusion proteins; solubility; circular dichroism; nuclear magnetic resonance; Structural biochemistry; Strukturbiokemi;

   Abstract : The number of gene products available for structural and functional study is increasing at an unprecedented rate as a result of the successful whole genome sequencing projects. Systematic structure determination of proteins on a genomic scale, called structural genomics, can significantly contribute to the field of protein science and to functional annotation of newly identified genes. READ MORE

6. 4. Genetic diversity of the heat labile (LT) and heat stable (ST) toxins of human enterotoxigenic Escherichia coli (ETEC): New insights into polymorphism, regulation, and gene transcription

   Author : Enrique Joffre; Göteborgs universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ETEC; diversity; bacterial enterotoxins; polymorphism; gene regulation; gene transcription; Diarrhoeal disease; bacterial infection;

   Abstract : Infection with enterotoxigenic Escherichia coli (ETEC) is a leading cause of diarrhea in children in developing countries and travelers to endemic regions. ETEC is a diverse pathogen, with a wide range of virulence factors including enterotoxins and more than 25 identified colonization factors (CFs). READ MORE

7. 5. Enhancing membrane and secretory protein production yields in Escherichia coli

   Author : Zhe Zhang; Jan-Willem de Gier; Claes von Wachenfeldt; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; recombinant protein production; Escherichia coli; membrane protein; secretory protein; strain engineering; Biochemistry; biokemi;

   Abstract : Proteins fulfill essential functions in living cells. To produce sufficient amounts of a protein is essential to study the structure and function of a protein, or to use it for medical purposes. Escherichia coli is a Gram-negative bacterium that is widely used for recombinant protein production. READ MORE