Search for dissertations about: "gene targeting"

Showing result 1 - 5 of 352 swedish dissertations containing the words gene targeting.

  1. 1. Conditional targeting of the PDGF-B gene

    Author : Maria Enge; Göteborgs universitet; Göteborgs universitet; Gothenburg University; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; gene targeting; Cre loxP; PDGF; pericytes; diabetic retinopathy; brain injury; neurons;

    Abstract : Platelet derived growth factors (PDGF) are known as mitogens for fibroblasts, smooth muscle cells (SMC) and glia cells in culture. Previous in vivo analyses have shown that PDGF-B is necessary for normal embryonic development and life after birth, but the relative importance of the various cellular sources of PDGF-B during development has not been clarified. READ MORE

  2. 2. Genetic re-targeting and de-targeting of adenovirus type 5 in order to create vectors for gene therapy

    Author : Susanna Myhre; Göteborgs universitet; Göteborgs universitet; Gothenburg University; []
    Keywords : Adenovirus; gene therapy; vector; re-targeting; de-targeting; fiber; pIX; affibody molecule; single chain antibody;

    Abstract : Gene therapy has been considered to be a revolutionary development in medicine, whereby the cause and not the symptoms of the disease would be treated. These expectations have as yet not been realized, mainly due to lack of suitable vectors. READ MORE

  3. 3. New Conditional Gene Targeting Methods : For Studying Neurotrophic Mechanisms in Selected Neuronal Populations

    Author : Jonas Lindeberg; Christer Betsholtz; Uppsala universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Neurosciences; Neurovetenskap; Neurology; Neurologi; Developmental Neurosciences; Medicinsk utvecklings- och neurobiologi;

    Abstract : Powerful techniques to manipulate the mouse genome have had a great impact on our understanding of biology. The major drawback with conventional transgenic methods is that the genetic alteration will be present in every cell of the animal, from conception and onwards. READ MORE

  4. 4. Targeting Infectious Disease : Structural and functional studies of proteins from two RNA viruses and Mycobacterium tuberculosis

    Author : Anna M. Jansson; T. Alwyn Jones; Sherry L. Mowbray; E. Yvonne Jones; Uppsala universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; RNA virus; coronavirus; alphacoronavirus; nsp1; TGEV; flavivirus; Modoc virus; NS5; methyltransferase; mRNA capping; Mycobacterium tuberculosis; tuberculosis; 1-deoxy-D-xylulose 5-phosphate reductoisomerase; DXR; fosmidomycin analogues; MEP pathway; drug development; xray-crystallography; Biology with specialization in Structural Biology; Biologi med inriktning mot strukturbiologi; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi; Biokemi; Biochemistry; Medicinal Chemistry; Läkemedelskemi;

    Abstract : The recent emergence of a number of new viral diseases as well as the re-emergence of tuberculosis (TB), indicate an urgent need for new drugs against viral and bacterial infections.Coronavirus nsp1 has been shown to induce suppression of host gene expression and interfere with host immune response. READ MORE

  5. 5. Chitosan Polyplexes as Non-Viral Gene Delivery Systems : Structure-Property Relationships and In Vivo Efficiency

    Author : Magnus Köping-Höggård; Per Artursson; Jean-Paul Behr; Uppsala universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmaceutics; gene therapy; gene delivery; non-viral; polyplex; chitosan; chitosan oligomers; polyethylenimine; plasmid DNA; lung; Galenisk farmaci; Pharmaceutics; Galenisk farmaci;

    Abstract : The subject of this thesis was to develop and optimize delivery systems for plasmid DNA (pDNA) based on biocompatible polymers, in particular chitosan, suitable for non-viral gene therapy. At the onset of this thesis, studies had reported conflicting results on the efficiency of chitosan-based gene delivery systems. READ MORE