Search for dissertations about: "glutamate oxidase"

Showing result 1 - 5 of 10 swedish dissertations containing the words glutamate oxidase.

2. 1. Creating Ultrafast Biosensors for Neuroscience

   Author : Yuanmo Wang; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; quantification; glucose; choline oxidase; gold nanoparticles; enzyme monolayer; enzymatic activity; Biosensor; adsorption; brain slice; enzyme stability; acetylcholine esterase; artificial cell; exocytosis; glucose oxidase; deformation; glutamate; glutamate oxidase; acetylcholine; amperometry;

   Abstract : Neuronal communication is the basis for all our brain function and relies on regulated exocytosis, a cell function that involves release of quantal amounts of neurotransmitters into the gap space between interconnected neurons to serve as chemical signals. To study exocytosis, which is a fast process that occurs on the timescale of sub-milliseconds to milliseconds, a toolbox of analytical methods has been developed where the electrochemical based techniques offer quantitative and sufficient high temporal recording speed. READ MORE

4. 2. Nitric Oxide Reductase from Paracoccus denitrificans : A Proton Transfer Pathway from the “Wrong” Side

   Author : Ulrika Flock; Pia Ädelroth; Simon de Vries; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; denitrification; nitric oxide reductase; heme copper oxidase superfamily; divergent member; proton transfer; electron transfer; single turnover; spectroscopy; periplasm; glutamate; proton pathway; Biochemistry; Biokemi; biokemi; Biochemistry;

   Abstract : Denitrification is an anaerobic process performed by several soil bacteria as an alternative to aerobic respiration. A key-step in denitrification (the N-N-bond is made) is catalyzed by nitric oxide reductase (NOR); 2NO + 2e- + 2H+ → N2O + H2O. READ MORE

5. 3. Structure and Function of Cytochrome c Oxidase

   Author : Gisela Brändén; Peter Brzezinski; Mårten Wikström; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; cytochrome c oxidase; proton pumping; proton transfer; electron transfer; X-ray crystallography; flow flash; Biochemistry; Biokemi;

   Abstract : Aerobic organisms, ranging from bacteria to humans, obtain their energy through the process of respiration. Electrons from the breakdown of food are transported through the membrane-bound enzyme complexes of the respiratory chain, to the terminal acceptor molecule, oxygen. READ MORE

6. 4. Enzyme-Based Amperometric Biosensors in Flow-Systems for Biomolecule Detection

   Author : Niklas Larsson; Centrum för analys och syntes; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Microdialysis; Liquid chromatography; Flow injection; Polyelectrolytes; Carbohydrate; Mediator; Dehydrogenase; Peroxidase; Oxidase; Enzyme; Amperometric detection; Carbon paste; Biosensor; Graphite electrode; On-line monitoring; Analytical chemistry; Analytisk kemi;

   Abstract : Amperometric enzyme based biosensors were designed, developed and characterised in flow systems. Solid graphite and carbon paste electrodes were used as support for enzyme immobilisation. Both mediated and direct electron transfers between redox enzymes and electrodes were utilised for biosensor construction, as well as coupled enzyme systems. READ MORE

7. 5. Development of Ultra-fast Biosensors for Detection of Non-electroactive Neurotransmitters

   Author : Yuanmo Wang; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; artificial cell; quantification; stability; deformation; dynamic light scattering; adsorption; amperometry; nanoparticle tracking analysis; enzyme monolayer; glucose oxidase; exocytosis; choline oxidase; acetylcholine; enzymatic activity; acetylcholine esterase; gold nanoparticles; Biosensor;

   Abstract : During neuronal communication neurotransmitters are released through the process exocytosis that occurs on the time scale of sub-milliseconds to milliseconds. Neuronal activity by neurotransmitters that are non-electroactive cannot easily be monitored due to limitations in the temporal resolutions of the sensor probes for these analytes. READ MORE