Search for dissertations about: "glutathione reductase"

Showing result 1 - 5 of 38 swedish dissertations containing the words glutathione reductase.

  2. 1. Glutathione reductase from erythrocytes : purification, characterization and identification of functional histidine and arginine residues of the enzyme

    Author : Vijayakumar Boggaram; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES;

    Abstract : This investigation aims at the development of a rapid, simple purification procedure for glutathione reductase and at an exploration of the chemical groups of the enzyme essential for activity.Glutathione reductase from porcine erythrocytes was purified to homogeneity by the use of affinity chromatography on 2’,5’ ADP-Sepharose 4B. READ MORE

  4. 2. Redesign of substrate specificity of glutathione transferase and glutathione reductase : Enzyme engineering by directed mutagenesis, phage-display selection and DNA shuffling

    Author : Lars O. Hansson; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Abstract : Human glutathione transferase (GST) A1-1 was expressed in fusion with the phage gene IIIproduct and a library of phage-displayed GST mutants was constructed by randomization of fouractive-site residues. Variant GSTs were isolated by mechanism-based phage display selection, using an immobilized transition-state analog of a nucleophilic aromatic substitution (SNAr) reaction. READ MORE

  5. 3. Purification and properties of glutathione reductase from liver

    Author : Inger Carlberg; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES;

    Abstract : The present investigation was undertaken in order to acheive an increased understanding of the molecular and catalytic properties of the flavin-containing enzyme glutathione reductase.Glutathione reductase from rat and calf liver was purified close to homogeneity, both by procedures involving standard techniques but also by procedures involving affinity chromatography on 2’, 5’-ADP Sepharose 4B. READ MORE

  6. 4. Dual targeting of glutathione reductase to mitochondria and chloroplasts

    Author : Charlotta Rudhe; Elzbieta Glaser; Ralf Bernad Klösgen; Stockholms universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; dual targeting; mitochondria; chloroplasts; import; Biochemistry; Biokemi;

    Abstract : As a consequence of the presence of both mitochondria and chloroplasts in plant cells there is a higher sorting requirement in a plant cell than that in a non-plant cell. Reflecting this, protein import to mitochondria and chloroplasts has been shown to be highly specific. READ MORE

  7. 5. Selenium in mammalian thioredoxin reductase

    Author : Liangwei Zhong; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : Thioredoxin reductase (TrxR) is a major enzyme in regulating intracellular redox status. The enzyme from several mammalian tissues has been purified, showing higher molecular weight and a much broader substrate specificity than E. coli TrxR, but the structural basis of these properties remained unknown. READ MORE