Search for dissertations about: "glutathione"

Showing result 1 - 5 of 281 swedish dissertations containing the word glutathione.

  1. 1. Glutathione transferases : molecular cloning, site-directed mutagenesis and structure-function studies

    Author : Gun Stenberg; Stockholms universitet; []

    Abstract : Three distinct glutathione transferases (GST) cDNA clones were obtained by screening of tumour cell line cDNA libraries. The cDNA clones were expressed in Escherichia coli and the enzymes characterized. Rat GST 8-8 is the only member in the multifunctional GST family for which a specific cellular function has been proposed. READ MORE

  2. 2. Glutathione transferases : studies on the structure and function of recombinant enzymes

    Author : Mikael Widersten; Uppsala universitet; []

    Abstract : .... READ MORE

  3. 3. Redesign of Alpha Class Glutathione Transferases to Study Their Catalytic Properties

    Author : Lisa O Nilsson; Ralf Morgenstern; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; glutathione transferase; heterologous expression; heterodimer; hydroxyalkenal; protein redesign; sequential design; dimer-interface interactions; enzyme kinetics; glutathione; dynamic C-terminus; viscosity dependence; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Abstract : A number of active site mutants of human Alpha class glutathione transferase A1-1 (hGST A1-1) were made and characterized to determine the structural determinants for alkenal activity. The choice of mutations was based on primary structure alignments of hGST A1-1 and the Alpha class enzyme with the highest alkenal activity, hGST A4-4, from three different species and crystal structure comparisons between the human enzymes. READ MORE

  4. 4. Microsomal glutathione transferase

    Author : Ralf Morgenstern; Stockholms universitet; []

    Abstract : A distinct glutathione transferase which can be activated up to eightfold by sulfhydryl reagents was discovered in rat liver microsomes. This enzyme was subsequently purified to apparent homogeneity in 40 % yield using column cromatography. READ MORE

  5. 5. Microsomal glutathione transferase : Species characteristics, catalysis and topology

    Author : Claes Andersson; Stockholms universitet; []

    Abstract : The membrane-bound detoxication enzyme microsomal glutathione transferase is localized predominantly in the liver of mammalian species, where it is present in high concentrations in the endoplasmic reticulum and the outer mitochondrial membrane. The enzyme conjugates a broad range of hydrophobic xenobiotics bearing reactive electrophilic centers to glutathione, which facilitates deactivation and excretion. READ MORE