Search for dissertations about: "glycoside hydrolase"

Showing result 6 - 10 of 41 swedish dissertations containing the words glycoside hydrolase.

  2. 6. In vitro and in vivo approaches in the characterization of XTH gene products

    Author : Nomchit Kaewthai; Harry Brumer; Jocelyn Rose; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Arabiodopsis thaliana; Hordeum vulgare; plant cell wall; xyloglucan; glycoside hydrolase family 16; xyloglucan endo-transglycosylase hydrolase gene family; xyloglucan endo-hydrolase; heterologous protein expression; Pichia pastoris; T-DNA insertion; in situ XET XEH assay; high-througput screening; Plant physiology; Växtfysiologi; Biochemistry; Biokemi; Functional genomics; Funktionsgenomik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Abstract : ABSTRACT The xyloglucan endo-transglycosylase/hydrolase (XTH) genes are found in all vascular and some nonvascular plants. The XTH genes encode proteins which comprise a subfamily of glycoside hydrolase (GH) family 16 in the Carbohydrate-Active enZYmes (CAZY) classification. READ MORE

  4. 7. Structural and biochemical insights into biosynthesis and degradation of N-glycans

    Author : Tom Reichenbach; Christina Divne; David Drew; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; N-glycan; biosynthesis; degradation; glycosyltransferase; glycoside hydrolase; biofuel; enzyme; membrane protein; Bioteknologi; Biotechnology;

    Abstract : Carbohydrates are a primary energy source for all living organisms, but importantly, they also participate in a number of life-sustaining biological processes, e.g. cell signaling and cell-wall synthesis. The first part of the thesis examines glycosyltransferases that play a crucial role in the biosynthesis of N-glycans. READ MORE

  5. 8. Investigation of genes and proteins involved in xylan biosynthesis

    Author : Anders Winzell; Ines Ezcurra; Johan Edqvist; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Populus; xylan biosynthesis; hemicellulose; glycosyltransferase; GT43; IRX; glycoside hydrolase; GH10; Xyn10A; CAZy; transcription factor; MYB; wood formation; secondary cell wall; Arabidopsis; Molecular biology; Molekylärbiologi;

    Abstract : Wood formation or xylogenesis is a fundamental process for so diverse issues as industry, shelter and a sustainable environment. Wood is comprised of secondary xylem, rigid large cells with thick cell walls that are lignified. READ MORE

  6. 9. Transglycosylation catalyzed by β-mannanases : Molecular aspects relevant for enzymatic synthesis

    Author : Johan Morrill; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; β-mannanase; glycoside hydrolase; enzymology; kinetics; MALDI-TOF MS; HPLC; transglycosylation; alcoholysis; enzyme engineering; protein structure; enzymatic synthesis;

    Abstract : β-Mannanases are a major type of enzyme involved in the hydrolysis and modification of β-mannans, a hemicellulose present in high amounts in e.g. softwoods such as spruce. The β-mannanases studied in this thesis belong to glycoside hydrolase (GH) families GH5 and GH26. READ MORE

  7. 10. Bench-Scale Production of Heterologous Proteins from Extremophiles- Escherichia coli and Pichia pastoris based expression systems

    Author : Santosh Ramchuran; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Glycoside hydrolase; Heterologous protein; High cell density cultivation Expression systems; Bioteknik; Biotechnology; Fed-batch;

    Abstract : Over the past few years considerable research attention has been assigned to extremophiles as sources of extremozymes due to their applicability in industrial processes, and the development of eco-friendly technologies. The establishment of efficient production strategies for heterologous proteins is an empirical process requiring broad background knowledge on available expression systems together with their major advantages and shortcomings. READ MORE