Search for dissertations about: "glycosynthase"

Found 5 swedish dissertations containing the word glycosynthase.

  1. 1. Synthesis of xyloglucan oligo- and polysaccharides with glycosynthase technology

    Author : Fredrika Gullfot; Harry Brumer; Tuula Teeri; Jerry Ståhlberg; Mats Martinelle; KTH; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; glycosynthase; xyloglucan; xyloglucan endo-transglycosylase; retaining glycoside hydrolase; xyloglucanase; polysaccharide synthesis; Bioengineering; Bioteknik; Enzyme engineering; Enzymteknik; Bioorganical synthesis; Bioorganisk syntes; Biomaterials; Biomaterial;

    Abstract : Xyloglucans are polysaccharides found as storage polymers in seeds and tubers, and as cross-linking glycans in the cell wall of plants. Their structure is complex with intricate branching patterns, which contribute to the physical properties of the polysaccharide including its binding to and interaction with other glycans such as cellulose. READ MORE

  2. 2. Production and engineering of a xyloglucan endo-transglycosylase from Populus tremula x tremuloides

    Author : Maria Henriksson; Tuula Teeri; KTH; []
    Keywords : AGRICULTURAL SCIENCES; LANTBRUKSVETENSKAPER; LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; xyloglucan endo-transglycosylase; retaining glycoside hydrolase; glycosynthase; Pichia pastoris; fed-batch fermentation; expanded-bed adsorption; Plant biotechnology; Växtbioteknik;

    Abstract : The aim of this work was to develop a production process for the enzyme xyloglucan endo-transglycosylase from Populus tremula x tremuloides (PttXET16-34). The natural transglycosylating activity of this enzyme has previously been employed in a XET-Technology. READ MORE

  3. 3. On the engineering of proteins: methods and applications for carbohydrate-active enzymes

    Author : Fredrika Gullfot; Harry Brumer; Antoni Planas; KTH; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; enzyme engineering; rational design; directed evolution; DNA shuffling; glycosynthase; xyloglucan; xyloglucan endo-transglycosylase; retaining glycoside hydrolase; xyloglucanase; carbohydrate binding module; polysaccharide synthesis; Bioengineering; Bioteknik;

    Abstract : This thesis presents the application of different protein engineering methods on enzymes and non-catalytic proteins that act upon xyloglucans. Xyloglucans are polysaccharides found as storage polymers in seeds and tubers, and as cross-linking glucans in the cell wall of plants. READ MORE

  4. 4. Thermostable glycosidases and glycosynthases as biocatalysts in green chemistry applications

    Author : Tania Pozzo; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Green chemistry; 3D sturcture; Glycosyl hydrolases; Glycosynthases; Alkyl glucosides; Oligosaccharides; Flavonoids;

    Abstract : The constant need to develop environmental friendly processes using renewable raw materials and to reduce harmful waste production motivates the present investigation focusing on biocatalyst development for enzymatic synthesis, a biotechnological approach in the field of green chemistry. At first, structural functional experiments were made for a β-glucosidase from T. READ MORE

  5. 5. Exploring the structure of oligo- and polysaccharides : Synthesis and NMR spectroscopy studies

    Author : Hanna Jonsson; Göran Widmalm; Antonio Molinaro; Stockholms universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Carbohydrates; NMR spectroscopy; synthesis; lipopolysaccharides; conformational studies; Organic chemistry; Organisk kemi; Organic Chemistry; organisk kemi;

    Abstract : A deeper understanding of the diversity of carbohydrates and the many applications of oligo- and polysaccharides found in nature are of high interest. Many of the processes involving carbohydrates affect our everyday life. This thesis is based on six papers all contributing to an extended perspective of carbohydrate property and functionality. READ MORE