Search for dissertations about: "green fluorescent protein GFP"

Showing result 1 - 5 of 54 swedish dissertations containing the words green fluorescent protein GFP.

  1. 1. Hydrophobic Interactions in Bioseparation - Applications of Modified Green Fluorescent Protein

    Author : Kristian Becker; Lunds universitet.; Lund University.; [2008]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Hydrophobic interaction chromatography; capillary electrochromatography; E. coli; isolation tags; haemoglobin; green fluorescent protein; retention prediction; genetic engineering; accessible surface area;

    Abstract : This thesis describes interactions that are salt-promoted and mainly based on the hydrophobicity of proteins. A series of variants of the green fluorescent protein (GFP) was first constructed by site-directed mutagenesis. Several tags with various properties were then genetically fused to the N-terminus of GFP. READ MORE

  2. 2. On bacterial formats in protein library technology

    University dissertation from Stockholm : KTH

    Author : Per-Åke Löfdahl; KTH.; [2009]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Affibody molecule; β-lactamase; combinatorial library; green fluorescent protein GFP ; protein engineering; protein fragment complementation assay PCA ; selection; TEVprotease; TNF-α; TECHNOLOGY Bioengineering; TEKNIKVETENSKAP Bioteknik; TECHNOLOGY Bioengineering Enzyme engineering; TEKNIKVETENSKAP Bioteknik Enzymteknik;

    Abstract : Millions of years of evolution have resulted in an immense number of different proteins, which participate in virtually every process within cells and thus are of utmost importance for allknown forms of life. In addition, there are several examples of natural proteins which have found use in applications outside their natural environment, such as the use of enzymes infood industry and washing powders or the use of antibodies in diagnostic, bioseparation or therapeutic applications. READ MORE

  3. 3. GFP as a tool to monitor membrane protein topology and overexpression in Escherichia coli

    University dissertation from Stockholm : Institutionen för biokemi och biofysik

    Author : David Drew; Stockholms universitet.; [2005]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; GFP; membrane protein; topology; overexpression; high-throughput; NATURAL SCIENCES Chemistry Biochemistry; NATURVETENSKAP Kemi Biokemi;

    Abstract : Membrane proteins are essential for life, and roughly one-quarter of all open reading frames in sequenced genomes code for membrane proteins. Unfortunately, our understanding of membrane proteins lags behind that of soluble proteins, and is best reflected by the fact that only 0. READ MORE

  4. 4. Metal Binding Tags - Characterisation, Use in Bioseparation and Applications of Green Fluorescent Fusion Proteins

    University dissertation from Pure and Applied Biochemistry, Lund University

    Author : Florent Bernaudat; Lunds universitet.; Lund University.; [2005]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; enzymology; E. coli; Immobilised metal ion affinity chromatography; Affinity tags; Green fluorescent protein; Surface plasmon resonance; Genetic engineering; Screening; Proteins; Immunoassay; Biotechnology; Bioteknik;

    Abstract : Most of the recombinant proteins produced nowadays are fused to affinity tags, in order to facilitate their purification through affinity chromato-graphy. Out the different tags available, poly-histidine tags are among the most commonly used and can help purification through immobilised metal ion affinity chromatography (IMAC). READ MORE

  5. 5. Towards subcellular localization of the human proteome using bioimaging

    University dissertation from Stockholm : KTH Royal Institute of Technology

    Author : Charlotte Stadler; KTH.; KTH.; [2012]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Antibody; antibody validation; automated image analysis; automated microscopy; cell line; confocal microscopy; fixation; green fluorescent protein GFP ; immunofluorescence IF ; organelle; protein expression; siRNA; subcellular localization;

    Abstract : Since the publication of the complete sequence of the human genome in 2003 there has been great interest in exploring the functions of the proteins encoded by the genes. To reveal the function of each and every protein, investigation of protein localization at the subcellular level has become a central focus in this research area, since the localization and function of a protein is closely related. READ MORE