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Showing result 1 - 5 of 61 swedish dissertations matching the above criteria.

  2. 1. Hydrophobic Interactions in Bioseparation - Applications of Modified Green Fluorescent Protein

    Author : Kristian Becker; Tillämpad biokemi; []
    Keywords : Hydrophobic interaction chromatography; capillary electrochromatography; E. coli; isolation tags; haemoglobin; green fluorescent protein; retention prediction; genetic engineering; accessible surface area;

    Abstract : This thesis describes interactions that are salt-promoted and mainly based on the hydrophobicity of proteins. A series of variants of the green fluorescent protein (GFP) was first constructed by site-directed mutagenesis. Several tags with various properties were then genetically fused to the N-terminus of GFP. READ MORE

  4. 2. On bacterial formats in protein library technology

    Author : Per-Åke Löfdahl; Per-Åke Nygren; Mikael Widersten; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Affibody molecule; β-lactamase; combinatorial library; green fluorescent protein GFP ; protein engineering; protein fragment complementation assay PCA ; selection; TEVprotease; TNF-α; Bioengineering; Bioteknik; Enzyme engineering; Enzymteknik;

    Abstract : Millions of years of evolution have resulted in an immense number of different proteins, which participate in virtually every process within cells and thus are of utmost importance for allknown forms of life. In addition, there are several examples of natural proteins which have found use in applications outside their natural environment, such as the use of enzymes infood industry and washing powders or the use of antibodies in diagnostic, bioseparation or therapeutic applications. READ MORE

  5. 3. GFP as a tool to monitor membrane protein topology and overexpression in Escherichia coli

    Author : David Drew; Jan-Willem de Gier; Gunnar von Heijne; Bert Poolman; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; GFP; membrane protein; topology; overexpression; high-throughput; Biochemistry; Biokemi;

    Abstract : Membrane proteins are essential for life, and roughly one-quarter of all open reading frames in sequenced genomes code for membrane proteins. Unfortunately, our understanding of membrane proteins lags behind that of soluble proteins, and is best reflected by the fact that only 0. READ MORE

  6. 4. Amyloid Beta – Biotherapy Target and Biotechnological Tool

    Author : Sebastian W. Meister; Stefan Ståhl; John Löfblom; Maarten Merkx; KTH; []
    Keywords : Alzheimer’s disease; amyloid beta; blood-brain barrier; green-fluorescent protein; intracellular assays; proteases; protease substrate specificity; protein aggregation; protein engineering.; Bioteknologi; Biotechnology;

    Abstract : Alzheimer’s disease is a progressive neurodegenerative disease characterized primarily by memory impairment and cognitive decline. As of 2020, an estimated 50 million people suffer from Alzheimer’s disease or related dementia and no disease-modifying treatment options are yet approved for clinical practice. READ MORE

  7. 5. Metal Binding Tags - Characterisation, Use in Bioseparation and Applications of Green Fluorescent Fusion Proteins

    Author : Florent Bernaudat; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; enzymology; E. coli; Immobilised metal ion affinity chromatography; Affinity tags; Green fluorescent protein; Surface plasmon resonance; Genetic engineering; Screening; Proteins; Immunoassay; Biotechnology; Bioteknik;

    Abstract : Most of the recombinant proteins produced nowadays are fused to affinity tags, in order to facilitate their purification through affinity chromato-graphy. Out the different tags available, poly-histidine tags are among the most commonly used and can help purification through immobilised metal ion affinity chromatography (IMAC). READ MORE