Search for dissertations about: "groove-binding"

Showing result 1 - 5 of 12 swedish dissertations containing the word groove-binding.

2. 1. Groove-binding Unsymmetrical Cyanine Dyes for DNA Applications

   Author : Maja Eriksson; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; polarized spectroscopy; bacteriophage T5; cryo-transmission electron microscopy; dynamic light scattering; DNA; intercalation; cyanine dyes; groove-binding; fluorescence spectroscopy; gel electrophoresis;

   Abstract : This work focuses on interactions between DNA and a group of newly developed unsymmetrical cyanine dyes. The aim of the work was to discover fluorescent dyes that can be used in biophysical studies of natural DNA where minimum perturbation of the DNA-helix is essential, where a slow dissociation rate of the dyes is needed and where the ionic strength is an important parameter. READ MORE

4. 2. DNA - drug interactions : groove binding versus intercalation

   Author : Ulrica Sehlstedt; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES;

   Abstract : .... READ MORE

5. 3. Detection of Biomolecules: Syntheses and Studies of Minor Groove Binding Unsymmetrical Cyanine Dyes and Chromogenic Enzyme Substrates

   Author : Jonas Karlsson; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; palladium-catalysed cross-couplings; optical spectroscopy; minor groove binding; DNA; cyanine dye; enzyme; intercalation; fluorescence; chromogenic;

   Abstract : Visualisation or detection of biomolecules is usually achieved by the use of fluorescent or highly coloured chromophores. The work presented in this thesis deals with the development of new chromogenic substrates for the detection of enzyme activity, and new DNA-binding fluorescent dyes for staining of DNA. READ MORE

6. 4. Minor groove binding unsymmetrical cyanine dyes : binding and dissociation studies in free solution and in electrophoresis gels

   Author : Maja Eriksson; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; electrophoresis; cyanide dyes; dissociation kinetics; minor groove binding; DNA; stopped-flow; unwinding; mobility shift assays;

   Abstract : .... READ MORE

7. 5. DNA-groove binding studied with polarized-light spectroscopy

   Author : Karl Jansen; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES;

   Abstract : .... READ MORE