Search for dissertations about: "heterologous protein expression"

Showing result 1 - 5 of 58 swedish dissertations containing the words heterologous protein expression.

  1. 1. Heterologous expression and characterization of xyloglucan endotransglycosylases from Populus tremula x tremuloides

    University dissertation from Stockholm : Bioteknologi

    Author : Åsa Kallas; KTH.; [2004]
    Keywords : xyloglucan endotransglycosylase; transglycosylation; hybrid aspen; cell expansion; heterologous expression; Pichia pastoris; N-glycosylation; X-ray crystallography;

    Abstract : Xyloglucan endotransglycosylases (XETs) are enzymes involvedin the reorganization of the primary cell wall. This thesisdescribes several aspects of XET16A from hybrid aspen (Populustremula x tremuloides). The gene was isolated from the hybridaspen cDNA library and the protein was expressed heterologouslyin the methylotrophic yeast Pichia pastoris. READ MORE

  2. 2. Bench-Scale Production of Heterologous Proteins from Extremophiles- Escherichia coli and Pichia pastoris based expression systems

    University dissertation from Santosh O. Ramchuran, Biotechnology (LTH), Lund University

    Author : Santosh Ramchuran; Lund University.; Lunds universitet.; [2005]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Glycoside hydrolase; Heterologous protein; High cell density cultivation Expression systems; Bioteknik; Biotechnology; Fed-batch;

    Abstract : Over the past few years considerable research attention has been assigned to extremophiles as sources of extremozymes due to their applicability in industrial processes, and the development of eco-friendly technologies. The establishment of efficient production strategies for heterologous proteins is an empirical process requiring broad background knowledge on available expression systems together with their major advantages and shortcomings. READ MORE

  3. 3. Laccases and Oxalate-Degrading Enzymes Heterologous Expression and Novel Applications in Lignocellulose Processing

    University dissertation from Department of Applied Microbiology, Lund University

    Author : Pierre Cassland; Lund University.; Lunds universitet.; [2004]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; virology; mycology; Mikrobiologi; virologi; mykologi; bacteriology; Microbiology; Lignocellulose Processing; Heterologous expression; Laccases; Oxalate-Degrading Enzymes; bakteriologi;

    Abstract : Lignocellulose constitutes a vast renewable resource for the production of, for example, paper and bioethanol. The potential of using laccase and oxalate-degrading enzymes in novel applications for the processing of lignocellulose was investigated in this work. READ MORE

  4. 4. Protein production, characterization and structure determination in structural genomics

    University dissertation from Bioteknologi

    Author : Esmeralda A. Woestenenk; KTH.; [2004]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biology; protein production; protein solubility; fusion tags; nuclear magnetic resonance; structure determination; Biologi; NATURAL SCIENCES Biology; NATURVETENSKAP Biologi;

    Abstract : This thesis covers the process from expression of a heterologous gene in Escherichia coli to structure determination of a protein by nuclear magnetic resonance (NMR) spectroscopy.The first part concerns structural genomics-related parallel screening studies on the effect of fusion tags (in particular the His tag) on protein solubility and the use of fusion tags in fast, parallel purification protocols intended for initial biophysical characterization of human proteins produced in E. READ MORE

  5. 5. On the way of making plants smell like moths - a synthetic biology approach

    University dissertation from Lund University, Faculty of Science, Department of Biology

    Author : Bao-Jian Ding; Lund University.; Lunds universitet.; [2014]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; moth pheromone; transcriptome; fatty acyl desaturase; fatty acyl reductase; acetyltransferase; chain shortening; chain elongation; heterologous expression; synthetic biology; plant expression;

    Abstract : Moth caterpillars are major agricultural pests in many parts of the world. In general female moths attract male mates with their pheromone over long distance. Most of the described moth pheromones belong to the same class of chemical compounds, fatty acid derivatives that are produced de novo in the pheromone gland. READ MORE