Search for dissertations about: "heterologous protein expression"

Showing result 16 - 20 of 62 swedish dissertations containing the words heterologous protein expression.

  1. 16. Fatty Acyl Reductases and Fatty Acyl Desaturases. Deciphering the biosynthesis of species-specific moth female sex pheromones from common fatty acids

    Author : Åsa Hagström; Biologiska institutionen; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Moth; pheromone; insect; evolution; fatty acyl desaturase; fatty acyl reductase; endoplasmic reticulum; fatty acid biosynthesis; GFP; heterologous expression; phylogeny; cell factory; lepidoptera;

    Abstract : Moths (Lepidoptera) are dependent on female produced pheromones, chemical signals, for attracting a mate. Pheromones are most commonly made up by saturated and/or unsaturated fatty acid derived alcohols, acetates, and/or aldehydes that are produced by a set of enzymes in the female pheromone gland. READ MORE

  2. 17. Plant UDP-glucose Pyrophosphorylase : Function and Regulation

    Author : Meng Meng; Leszek Kleczkowski; Javier Pozueta-Romero; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; UDP-glucose pyrophosphorylase; carbohydrate metabolism; in vivo regulation; T-DNA knockout; heterologous expression; isozyme; mutagenesis; kinetic property; oligomerization; Plant physiology; Växtfysiologi;

    Abstract : UDP-glucose pyrophosphorylase (UGPase) is an important enzyme of carbohydrate metabolism in all living organisms. The main aim of this thesis was to investigate the function and regulation of plant UGP genes as well as the UGPase proteins. READ MORE

  3. 18. Microsomal glutathione transferase : catalysis, in vitro mutagenesis and heterologous expression

    Author : Rolf Weinander; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : Microsomal glutathione transferase is a membrane-bound member of the glutathione transferases, a family of multifunctional enzymes involved in the cellular detoxification of xenobiotics and reactive endogenous compounds formed during oxidative stress. Rat liver microsomal glutathione transferase is a trimeric protein with a molecular mass of 17. READ MORE

  4. 19. Functional genomics at the interface of protein expression and biophysical analysis

    Author : Xiaoqiu Wu; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : Protein characterization is central to any functional genomics effort, hence protein expression and purification play fundamental roles in these initiatives. In this study strategies for protein expression in E. coli were analysed, and several parameters were investigated respect to high-level expression of functional proteins. READ MORE

  5. 20. Alpha-2 Adrenergic Receptors and Signal Transduction : Effector Output in Relation to G-Protein Coupling and Signalling Cross-Talk

    Author : Johnny Näsman; Peter Evans; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Physiology and anatomy; Adrenergic; receptor; G-protein; adenylyl cyclase; cAMP; calcium; cross-talk; Fysiologi och anatomi; Physiology and pharmacology; Fysiologi och farmakologi; Physiology; fysiologi;

    Abstract : The alpha-2 adrenergic receptor (α2-AR) subfamily includes three different subtypes, α2A-, α2B- and α2C-AR, all believed to exert their function through heterotrimeric Gi/o-proteins. The present study was undertaken in order to investigate subtype differences in terms of cellular response and to explore other potential signalling pathways of α2-ARs. READ MORE