Search for dissertations about: "high throughput screening assays"

Showing result 1 - 5 of 36 swedish dissertations containing the words high throughput screening assays.

  2. 1. High-throughput screening using multicellular tumor spheroids to reveal and exploit tumor-specific vulnerabilities

    Author : Wojciech Senkowski; Mårten Fryknäs; Rolf Larsson; Peter Nygren; Mats Gustafsson; Krister Wennerberg; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; spheroids; high-throughput screening; nitazoxanide; OXPHOS; gene expression profiling; statins; drug repositioning; 3D cell culture; quiescent cells; Medical Science; Medicinsk vetenskap;

    Abstract : High-throughput drug screening (HTS) in live cells is often a vital part of the preclinical anticancer drug discovery process. So far, two-dimensional (2D) monolayer cell cultures have been the most prevalent model in HTS endeavors. However, 2D cell cultures often fail to recapitulate the complex microenvironments of in vivo tumors. READ MORE

  4. 2. Toxicometabolomics and biotransformation product screening in single zebrafish embryos

    Author : Anton Ribbenstedt; Jonathan P. Benskin; Magnus Breitholtz; Elena Gorokhova; Emma Schymanski; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Non-target; REACH; Orbitrap; single embryo; zebrafish embryo; metabolism; high throughput; hydroxylation; glucuronidation; metabolite screening; phase I; phase II; pharmaceutical; structure elucidation; conjugation; propranolol; beta blocker; Non-targeted; toxicometabolomics; Orbitrap; single embryo; 3R; metabolomics; MoA; mode of action; zebrafish embryo; carbamazepine; biotransformation; Applied Environmental Science; tillämpad miljövetenskap;

    Abstract : Over the last decade environmental agencies worldwide have escalated their work to phase out animal testing for the purposes of chemical regulation. Meanwhile the number of commercially available chemicals and the requirements for hazard assessments have both increased, creating a large need for substitution of traditional in vivo assays with in vitro tests. READ MORE

  5. 3. High Content Analysis of Proteins and Protein Interactions by Proximity Ligation

    Author : Karl-Johan Leuchowius; Ola Söderberg; Ulf Landegren; Paul Goldsmith; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; in situ; proximity ligation; flow cytometry; high content screening; rolling circle amplification; in situ PLA; single-cell; single-molecule; protein interactions; drug screening; post-translational modifications; Molecular medicine; Molekylär medicin; Molekylär medicin; Molecular Medicine;

    Abstract : Fundamental to all biological processes is the interplay between biomolecules such as proteins and nucleic acids. Studies of interactions should therefore be more informative than mere detection of expressed proteins. Preferably, such studies should be performed in material that is as biologically and clinically relevant as possible, i.e. READ MORE

  6. 4. Towards novel antibacterials : Synthesis and identification of natural product inspired inhibitors of Chlamydia trachomatis and development of chemical probes targeting virulence of Pseudomonas aeruginosa

    Author : Michael Saleeb; Mikael Elofsson; Anna Linusson Jonsson; Andreas Kirschning; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Antibacterials; antibiotics; small molecules; natural products; benzofuran; dihydrobenzofuran; the type III secretion system; Pseudomonas aeruginosa; Chlamydia trachomatis; phenotypic screening; high-throughput screening; surface plasmon resonance; drug discovery; bacterial toxins; enzyme inhibitors; organisk kemi; Organic Chemistry;

    Abstract : Antibiotic resistance has evolved significantly to become one of the serious threats to public health today. Yet, the pipeline of new antibiotics is drying up and is lagging behind the challenging needs. READ MORE

  7. 5. Sensitivity, Noise and Detection of Enzyme Inhibition in Progress Curves

    Author : Omar Gutierrez Arenas; Helena Danielson; Athel Cornish-Bowden; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Progress curves; limit of detection; Z -factor; primary screening; enzyme inhibition; error structure; sensitivity analysis; High-throughput screning; Biokemi;

    Abstract : Starting with the development of an enzymatic assay, where an enzyme in solution hydrolysed a solid-phase bound peptide, a model for the kinetics of enzyme action was introduced. This model allowed the estimation of kinetic parameters and enzyme activity for a system that has the peculiarity of not being saturable with the substrate, but with the enzyme. READ MORE