Search for dissertations about: "hplc method development thesis"

Showing result 11 - 15 of 38 swedish dissertations containing the words hplc method development thesis.

  2. 11. Hydrolases as Catalysts for Green Chemistry and Industrial Applications - Esterase, Lipase and Phytase

    Author : Yasser Gaber; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; HPLC-EAT; sustainable environment; mutation; metallo-enzyme; phytate; b-propeller; biodegraqdable; metrics; green chemistry; Novozym 435; CalB; solvent-free; kinetic resolution; Biocatalysis; rational design; PLE; esterase; clopidogrel; enantioselectivity;

    Abstract : The use of enzymes in industrial applications has been recognised for providing clean processes with minimal impact on the environment. This thesis presents studies on engineering of enzymes and enzyme-based processes in the light of green chemistry and environmental sustainability, and focuses on three hydrolases: esterase, lipase and phytase. READ MORE

  4. 12. Chemical labelling methods for gel free based proteomics

    Author : Åsa Wåhlander; Institutionen för immunteknologi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biotechnology; Bioteknik; Biokemisk teknik; Biochemical technology; 2D-HPLC; isotopes; labelling; proteomics; mass spectrometry;

    Abstract : In this thesis different aspects of gel free procedures for mass spectrometry based proteome analysis are addressed, with emphasis on labelling techniques. It is based on three original articles where the effects imposed onto peptides upon modification are investigated, and the development of a new type of labelling strategy is described. READ MORE

  5. 13. Progress of Weak Affinity Chromatography as a Tool in Drug Development

    Author : Elinor Meiby; Sten Ohlson; Manfred Raida; Linnéuniversitetet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; weak affinity chromatography; fragment based drug discovery; fragment screening; thrombin; trypsin; cyclin G-associated kinase; Hsp90; Pin1; cyclooxygenase; lipodisks; Biokemi; Biochemistry;

    Abstract : Weak Affinity Chromatography (WAC) is a technology that was developed to analyse weak (KD > 10-5 M) although selective interactions between biomolecules. The focus of this thesis was to develop this method for various applications in the drug development process.Fragment Based Drug Discovery is a new approach in finding new small molecular drugs. READ MORE

  6. 14. High-performance liquid chromatography for analysis of 32P-postlabeled DNA adducts

    Author : Magnus Zeisig; Karolinska Institutet; Karolinska Institutet; []
    Keywords : DNA adducts; 32P-postlabeling; HPLC; method development;

    Abstract : The formation of DNA adducts, i.e. the covalent binding of chemicals and chemical groups to DNA, is believed to be an important step in chemical carciogenesis. DNA adducts are usually formed at very low levels which requires very sensitive methods to analyze them. READ MORE

  7. 15. Determination of folate for food composition data : development and improvement of analytical methods

    Author : Hanna Sara Strandler; Sveriges lantbruksuniversitet; Sveriges lantbruksuniversitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES;

    Abstract : Folate is B vitamin that is required for maintaining normal blood status. Recommendations on daily intake are based on information on blood folate status in relation to folate intake and bioavailability. Folate intake from a diet is evaluated by folate content in the food consumed. READ MORE