Search for dissertations about: "iPSC"

Showing result 1 - 5 of 17 swedish dissertations containing the word iPSC.

  1. 1. Genetics of Two Mendelian Traits and Validation of Induced Pluripotent Stem Cell (iPSC) Technology for Disease Modeling

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Doroteya Raykova; Niklas Dahl; Ann Nordgren; [2015]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Disease modeling; Mendelian disorders; iPSC; Whole exome sequencing; Transcriptome sequencing; Medical Science; Medicinsk vetenskap;

    Abstract : Novel technologies for genome analysis have provided almost unlimited opportunities to uncover structural gene variants behind human disorders. Whole exome sequencing (WES) is especially useful for understanding rare Mendelian conditions, because it reduces the requirements for a priori clinical data, and can be applied on a small number of patients. READ MORE

  2. 2. Device Bonding and Antibody Binding: Developments for On-Chip Differentiation of Induced Pluripotent Stem Cells into Hepatocytes

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Philip Dalsbecker; Göteborgs universitet.; Gothenburg University.; [2018]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Microfluidics; liver-on-a-chip; organ-on-chip; PDMS; oxygen plasma; immunofluorescence; fluorescence microscopy; hepatocytes; iPSC; induced pluripotent stem cells; differentiation; on-chip differentiation; definitive endoderm;

    Abstract : Abstract This thesis describes the bonding optimization of a liver-on-a-chip microfluidic device for evaluation of cultured induced pluripotent stem cell (iPSC)-derived definitive endoderm (DE) cells during their targeted differentiation into mature hepatocytes. Furthermore, it describes the optimization of a number of antibody assays for use during said differentiation, for the purpose of studying the cellular phenotype. READ MORE

  3. 3. Induced pluripotent stem cell (iPSC) modelling for the identification of mechanisms behind neurodevelopmental disorders

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Loora Laan; Niklas Dahl; Jens Schuster; Joakim Klar; Anna Falk; Jared Sterneckert; [2020]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Induced pluripotent stem cells; Neurogenesis; Neural differentiation; Down syndrome; Dravet syndrome; Ataxia; Medical Cell Biology; Medicinsk cellbiologi;

    Abstract : Human induced pluripotent stem cells (iPSCs) have opened new possibilities to recapitulate disease mechanisms and to model disorders in vitro. In the studies presented here, iPSCs were established to model neural differentiation in Down syndrome (DS), caused by trisomy for chromosome 21 (T21); Dravet syndrome (DRS), caused by variants in the SCN1A gene; and an ataxia syndrome, caused by a variant in the NFASC gene. READ MORE

  4. 4. Human iPSC derived neural cells as models of brain development and as tools in pharmaceutical drug discovery

    University dissertation from Stockholm : Karolinska Institutet, Dept of Neuroscience

    Author : Anders Lundin; Karolinska Institutet.; Karolinska Institutet.; [2018]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES;

    Abstract : Human brain evolution has resulted in a cognitive superiority compared to all other animals. Unique cortical structures and expanding progenitor populations have been associated with the possibility for developing a highly folded neocortex and expanded surface area, which is linked to cognitive function. READ MORE

  5. 5. The Effects of Genetic and Epigenetic Variation on Human Pluripotent Stem Cell Differentiation

    University dissertation from Lund University: Faculty of Medicine

    Author : Roksana Moraghebi; [2017]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES;

    Abstract : Human pluripotent stem cells (PSCs) are widely used for studying embryonic development, disease modelling, drug discovery and cell therapy development. Using human PSCs as a model has significantly contributed to our understanding of embryonic development and elucidating novel pathological mechanisms as well as developing new drugs. READ MORE