Search for dissertations about: "immobilised cells"

Showing result 1 - 5 of 25 swedish dissertations containing the words immobilised cells.

  1. 1. Porous Silicon Microbioreactors - Applications and Technology

    Author : Martin Bengtsson; Avdelningen för Biomedicinsk teknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Elektroteknik; Electrical engineering; immobilised cells; enzyme reactor; micro fluidics; Microreactor; porous silicon;

    Abstract : Microfluidic systems have become one of the most interesting fields in the research of chemical analysis. Miniaturized systems exhibit numerous practical advantages when compared with traditional batch-scale synthesis such as lower sample volumes, increased control of the reactions and higher sample throughput. READ MORE

  2. 2. Engineering of thermometric biosensors and characterisation of redox enzymes for improved performance of biosensors/biofuel cells

    Author : Maria Yakovleva; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Flow injection analysis; thermometric calorimetric biosensor; enzyme thermistor; immobilised enzyme reactor; amperometric biosensor; enzymatic biofuel cell; bioanode; pyranose dehydrogenase; cellobiose dehydrogenase; direct electron transfer; mediated electron transfer; matrix-assisted laser desorption ionization; glycosite mapping;

    Abstract : The present work is mainly focused on the development of enzyme-based bioanalytical devices, which can further be applied as analytical tools for industrial, medical and environmental applications. The first part of the work deals with the practical applications of thermometric biosensors for determination of various sugars (fructose and lactose) in a real samples. READ MORE

  3. 3. Strategies for facilitated protein recovery after recombinant production in Escherichia coli

    Author : My Hedhammar; Sophia Hober; Alois Jungbauer; KTH; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ion-exchange chromatography; protein A; Z; Zbasic; Zacid; fusion protein; proteolytic cleavage; immobilised protease; flow cytometry; inclusion bodies; solid-phase refolding; Molecular biology; Molekylärbiologi;

    Abstract : The successful genomic era has resulted in a great demand for efficient production and purification of proteins. The main objective of the work described in this thesis was to develop methods to facilitate recovery of target proteins after recombinant production in Escherichia coli. READ MORE

  4. 4. Regenerative effects of mesenchymal stem cell-derived exosomes

    Author : Xiaoqin Wang; Göteborgs universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; aging; cell adhesion; cell-material interaction; exosomes; extracellular vesicles; mesenchymal stem cells; osteogenic differentiation; proliferation; regeneration; titanium;

    Abstract : Mesenchymal stem cells (MSCs) play pivotal roles for bone regeneration by virtue of their osteogenic differentiation ability and immunomodulatory capacity. Recently, secretion of exosomes/extracellular vesicles (EVs) has been suggested as a new mechanism of MSC-based therapy. READ MORE

  5. 5. Heterogeneous electron transfer studies with ligninolytic redox enzymes and living bacteria. Applications in biosensors and biofuel cells

    Author : Vasile Coman; Centrum för analys och syntes; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; cellobiose dehydrogenase; direct electron transfer; mediated electron transfer; self-assembled monolayer; flow injection analysis; amperometric biosensor; E. coli; succinate:quinone reductase; biofuel cell; B. subtilis; whole-cell biosensor;

    Abstract : The catalytic properties and the inter-domain electron transfer of cellobiose dehydrogenase (CDH) from the ascomycete fungus Myriococcum thermophilum adsorbed on graphite and thiol (SAM) modified gold electrodes were investigated using cyclic voltammetry, flow injection amperometry and UV-Vis spectroelectrochemistry. The fabrication and characterisation of a noncompartmentalised, mediator and cofactor free glucose–oxygen biofuel cell well-operating in glucose-containing buffers and human blood serum was performed. READ MORE