Search for dissertations about: "immunofluorescence staining"

Showing result 1 - 5 of 41 swedish dissertations containing the words immunofluorescence staining.

  2. 1. Antibody-based subcellular localization of the human proteome

    Author : Marie Skogs; Emma Lundberg; Hans Blom; Cecilia Williams; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Human proteome; Subcellular localization; Organelles; Immunofluorescence; Fixation; Permeabilization; Antibody validation; Bioteknologi; Biotechnology;

    Abstract : This thesis describes the use of antibodies and immunofluorescence for subcellular localization of proteins. The key objective is the creation of an open-source atlas with information on the subcellular location of every human protein. READ MORE

  4. 2. Device Bonding and Antibody Binding: Developments for On-Chip Differentiation of Induced Pluripotent Stem Cells into Hepatocytes

    Author : Philip Dalsbecker; Göteborgs universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Microfluidics; liver-on-a-chip; organ-on-chip; PDMS; oxygen plasma; immunofluorescence; fluorescence microscopy; hepatocytes; iPSC; induced pluripotent stem cells; differentiation; on-chip differentiation; definitive endoderm;

    Abstract : Abstract This thesis describes the bonding optimization of a liver-on-a-chip microfluidic device for evaluation of cultured induced pluripotent stem cell (iPSC)-derived definitive endoderm (DE) cells during their targeted differentiation into mature hepatocytes. Furthermore, it describes the optimization of a number of antibody assays for use during said differentiation, for the purpose of studying the cellular phenotype. READ MORE

  5. 3. Generation and characterization of antibodies for proteomics research

    Author : Karin Larsson; Sophia Hober; John McCafferty; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody; antibody validation; immunohistochemistry; immunofluorescence; tissue microarray; Western blot; epitope mapping; antigen design; Bioengineering; Bioteknik;

    Abstract : Specific antibodies are invaluable tools for proteomics research. The availability of thoroughly validated antibodies will help to improve our understanding of protein expression, localization and function; fundamental processes and features of all living organisms. READ MORE

  6. 4. The nervous system of box jellyfish: A surprisingly complex system in a simple animal

    Author : Linda Parkefelt; Biologiska institutionen; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; proliferating cell nuclear antigen; arginine vasotocin; RFamide; immunofluorescence; visual system; nervous system; Tripedalia cystophora; Cnidaria; box jellyfish;

    Abstract : The four surprisingly complex visual sensory organs of cubomedusae, the rhopalia, contain each six eyes of four morphologically different types, two of which are camera-type eyes. This means that the evolutionary basal nervous system of cubomedusae must process the visual signals received from a total of 24 rhopalial eyes. READ MORE

  7. 5. Immunofluorescence studies on the cell cycle expression of cyclin A and cyclin E

    Author : Fredrik Erlandsson; Karolinska Institutet; Karolinska Institutet; []
    Keywords : Cell cycle regulation; cyclin A; cyclin E; immunofluorescence staining;

    Abstract : Cell cycle progression is tightly regulated in normal eucaryotic cells. Cancer is caused by the transformation of cells, and is always associated with defects in the cell cycle regulatory machinery. READ MORE