Search for dissertations about: "in situ PLA"

Showing result 16 - 20 of 30 swedish dissertations containing the words in situ PLA.

  2. 16. Proximity Ligation Assay for High Performance Protein Analysis in Medicine

    Author : Gucci Jijuan Gu; Ulf Landegren; Thomas Joos; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; protein; post-translational modification; interactions; protein domain; western blotting; MARK; tau; AD; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Abstract : High quality reagents are preconditions for high performance protein analyses. But despite progress in some techniques, e.g. mass spectrometry, there is still a lack of affinity-based detection techniques with enhanced precision, specificity, and sensitivity. READ MORE

  4. 17. Non-canonical TGFb signaling pathways in prostate cancer

    Author : Jie Song; Marene Landström; Staffan Strömblad; Umeå universitet; []
    Keywords : TGFβ; TβRI; TRAF6; ubiquitination; APPL1; p85; AKT; PKCζ;

    Abstract : Prostate cancer is the second leading cause of cancer-related death in men in the Western world. Deregulation of transforming growth factor β (TGFβ) signaling pathway is frequently detected in prostate cancer and contributes to tumor growth, migration, and invasion. READ MORE

  5. 18. Development of DNA-based methods for analysis of protein interactions

    Author : Axel Klaesson; Ola Söderberg; Mats Nilsson; Cristina Al-Khalili Szigyarto; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; In situ proximity ligation assay PLA ; rolling circle amplification RCA ; Hybridization chain reaction HCR ; proxHCR; Oligonucleotide design; Protein-protein interactions; Post-translational modifications.; Farmaceutisk vetenskap; Pharmaceutical Science; Molekylär cellbiologi; Molecular Cellbiology;

    Abstract : In situ proximity ligation assay (PLA) is a method for detection of protein interactions, post-translational modifications (PTMs) and individual proteins that allows information about their localization in a cell or tissue to be extracted. The method is based on oligonucleotide-conjugated antibodies (proximity probes) that upon binding of two epitopes in close proximity give rise to an amplifiable DNA circle. READ MORE

  6. 19. Analyses of Akt and PDGF receptor expression and activation in human tumors

    Author : Janna Paulsson; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : These studies aimed at developing methodology for detection of proteins involved in cancer and the evaluation of their prognostic significance in human tumors. Signal transduction profiling of tumors are becoming increasingly interesting based on the accumulating evidence of its prognostic and drug response-predicative value. READ MORE

  7. 20. Losing connections in Alzheimer disease : the amyloid precursor protein processing machinery at the synapse

    Author : Jolanta L Lundgren; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : Synaptic degeneration is one of the earliest characteristics of Alzheimer disease (AD). The amyloid β-peptide (Aβ) plays a critical role in the pathology of AD and therefore thorough understanding of its production and functions is of outmost importance. READ MORE