Search for dissertations about: "in situ PLA"

Showing result 6 - 10 of 30 swedish dissertations containing the words in situ PLA.

  2. 6. Molecular Tools for Detection and Characterization of Proteins and Extracellular Vesicles in Health and Disease

    Author : Ehsan Manouchehri Doulabi; Masood Kamali-Moghaddam; Lucia Mincheva-Nilsson; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; High-resolution mass spectrometry; Proximity ligation assay; Proximity extension assay; Thymidine kinase 1; in situ PLA; High-throughput.; Molekylär medicin; Molecular Medicine;

    Abstract : Detecting molecules involved in cancer is critical for cancer research and diagnostics. To achieve this goal, sensitive protein detection is essential to improving the chances of finding, verifying, validating and developing valuable biomarkers. READ MORE

  4. 7. Method development for the analysis of protein interactions

    Author : Despoina Kermpatsou; Ola Söderberg; Maréne Landström; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; in situ; proximity ligation assay PLA ; strand displacement; proximity-dependent initiation of hybridisation chain reaction proxHCR ; protein complexes; rolling circle amplification RCA ; MolBoolean; vitamin D; 1; 25D3-MARRS receptor; PDIA3; ERp57; CYP24A1; c-Myc; prostate cancer; Molekylär cellbiologi; Molecular Cellbiology; Molekylär medicin; Molecular Medicine;

    Abstract : Biological processes take place through interactions between macromolecules, such as nucleic acids and proteins. It is, therefore, fundamental to understand the functions of proteins and how they form complexes in order to carry out their role. READ MORE

  5. 8. Advancing DNA-based proximity methods

    Author : Mattias Leino; Ola Söderberg; John Löfblom; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; in situ; proximity dependent intiation of hybridization chain reaction; hybridization chain reaction; Oligonucleotide design; proximity ligation assay; protein interaction; microscopy; purification; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Abstract : Cellular functions are governed by intricate chains of interactions between proteins. In order to properly understand cellular biology one must look into, not only protein function, but also its interacting network. READ MORE

  6. 9. Improvements and Applications of in situ Proximity Ligation Assays

    Author : Karin Grannas; Ola Söderberg; Christer Wingren; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molekylär medicin; Molecular Medicine;

    Abstract : The cells building up the human body is in constant communication with each other. This communication is done through large complex networks of signaling pathways for inter- and intracellular signal transduction. The signaling activity regulates many important processes, for example cell death, proliferation and differentiation. READ MORE

  7. 10. Detection and Sequencing of Amplified Single Molecules

    Author : Rongqin Ke; Mats Nilsson; Ulf Landegren; Radoje Drmanac; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; padlock probes; proximity ligation assay; rolling circle amplification; circle-to-circle amplification; single molecule detection; amplified single molecules; sequencing; in situ; single cell; Molecular Biotechnology; Molekylär bioteknik;

    Abstract : Improved analytical methods provide new opportunities for both biological research and medical applications. This thesis describes several novel molecular techniques for nucleic acid and protein analysis based on detection or sequencing of amplified single molecules (ASMs). READ MORE