Search for dissertations about: "in situ sequencing"

Showing result 11 - 15 of 85 swedish dissertations containing the words in situ sequencing.

  2. 11. Single Molecule Detection : Microfluidic Automation and Digital Quantification

    Author : Malte Kühnemund; Mats Nilsson; Ulf Landegren; Sten Linnarsson; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; single molecule; digital; rolling circle amplification; magnetic particle; padlock probe; microfluidics; resistive pulse sensing; lab on chip; mobile phone microscopy; enrichment; sequencing;

    Abstract : Much of recent progress in medical research and diagnostics has been enabled through the advances in molecular analysis technologies, which now permit the detection and analysis of single molecules with high sensitivity and specificity. Assay sensitivity is fundamentally limited by the efficiency of the detection method used for read-out. READ MORE

  4. 12. iLocks: a novel tool for RNA assays with improved specificity

    Author : Tomasz Krzywkowski; Mats Nilsson; Gunnar von Heijne; Weng-Onn Lui; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; RNA; miRNA; non-coding RNA; padlock probes; rolling circle amplification; invader; single cell; in situ; adenovirus; virology; diagnostics; Biochemistry; biokemi;

    Abstract : The Central Dogma of molecular biology describes a framework for how genetic information is transferred in cells, placing RNA as a messenger between DNA and translated proteins. During the last years, interest in RNA research has grown tremendously due to the increasing understanding and recognition of the importance of RNA in regulation of gene expression, biochemical catalysis, and genome integrity surveillance. READ MORE

  5. 13. Analysis of signaling pathway activity in single cells using the in situ Proximity Ligation Assay

    Author : Linda Arngården; Ola Söderberg; Niclas Karlsson; Uppsala universitet; []
    Keywords : cell signaling; Wnt; Hippo; TGFB; Molekylär medicin; Molecular Medicine;

    Abstract : A cell that senses signals from its environment uses proteins for signal transduction via post translational modifications (PTMs) and protein- protein interactions (PPIs) from cell membrane into the nucleus where genes controlling cell proliferation, differentiation and apoptosis can be turned on or off, i.e. READ MORE

  6. 14. Computational Methods for Image-Based Spatial Transcriptomics

    Author : Axel Andersson; Carolina Wählby; Anna Klemm; Filip Malmberg; Karl Åström; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Spatial omics; Spatial transcriptomics; Spatial biology; In Situ Sequencing; Visualization; Spatial statistics; Fluorescence microscopy; Computerized Image Processing; Datoriserad bildbehandling;

    Abstract : Why does cancer develop, spread, grow, and lead to mortality? To answer these questions, one must study the fundamental building blocks of all living organisms — cells. Like a well-calibrated manufacturing unit, cells follow precise instructions by gene expression to initiate the synthesis of proteins, the workforces that drive all living biochemical processes. READ MORE

  7. 15. Inflammation and immunothrombosis in cerebral cavernous malformation : Novel molecular targets for the treatment of an incurable disease

    Author : Maria Ascención Globisch; Peetra Magnusson; Elisabetta Dejana; Marie Jeansson; Anna Randi; Uppsala universitet; []
    Keywords : Cavernoma; Inflammation; Coagulation; Blood-brain-barrier; Blood; Hypoxia; Propranolol; Biologi med inriktning mot molekylär immunologi; Biology with specialization in Molecular Immunology;

    Abstract : Cerebral cavernous malformation (CCM) is a vascular disease that causes mulberry-like lesions (cavernomas) in the central nervous system (CNS). Cavernomas are fragile, leaky and prone to rupture which may cause symptoms such as epileptic seizures, focal neurological deficits and hemorrhagic strokes. CCM lesions can appear sporadically in 0. READ MORE