Search for dissertations about: "inclusion bodies"

Showing result 1 - 5 of 29 swedish dissertations containing the words inclusion bodies.

  1. 1. Analytical tools for monitoring and control of fermentation processes

    Author : Heléne Sundström; Sven-Olof Enfors; Carl-Fredrik Mandenius; KTH; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Escherichia coli; flow cytometry; software sensors; viability; inclusion bodies; biomass; specific growth rate; stress; population heterogeneity; process analytical technology.; Biochemical process engineering; Biokemisk och bioteknisk processteknik;

    Abstract : The overall objective of this work has been to adopt new developments and techniques in the area of measurement, modelling and control of fermentation processes. Flow cytometry and software sensors are techniques which were considered ready for application and the focus was set on developing tools for research aiming at understanding the relationship between measured variables and process quality parameters. READ MORE

  2. 2. Strategies for facilitated protein recovery after recombinant production in Escherichia coli

    Author : My Hedhammar; Sophia Hober; Alois Jungbauer; KTH; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ion-exchange chromatography; protein A; Z; Zbasic; Zacid; fusion protein; proteolytic cleavage; immobilised protease; flow cytometry; inclusion bodies; solid-phase refolding; Molecular biology; Molekylärbiologi;

    Abstract : The successful genomic era has resulted in a great demand for efficient production and purification of proteins. The main objective of the work described in this thesis was to develop methods to facilitate recovery of target proteins after recombinant production in Escherichia coli. READ MORE

  3. 3. Structural and physicochemical studies on metal and porphyrin binding by the Bacillus subtilis ferrochelatase

    Author : Mattias Hansson; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; metal specificity; metal binding; porphyrin metallation; time resolved crystallography; fluorescence quenching; X-ray structure; inclusion bodies; Bacillus subtilis; heme biosynthesis; ferrochelatase; hemH;

    Abstract : The enzyme ferrochelatase (EC 4.99.1.1) is the terminal enzyme in the biosynthetic pathway of heme b and catalyzes the insertion of Fe(II) into protoporphyrin IX. READ MORE

  4. 4. Interaction engineered three-helix bundle domains for protein recovery and detection

    Author : Tove Alm; Sophia Hober; Rainer Hahn; KTH; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ABD; Zbasic; albumin; protein engineering; phage display; staphylococcal display; inclusion bodies; refolding; proteomics; orthogonal affinity purification; Bioengineering; Bioteknik; Immunology engineering; Immunteknik;

    Abstract : HTML clipboard The great advances in DNA technology, e.g. sequencing and recombinant DNA techniques, have given us the genetic information and the tools needed to effectively produce recombinant proteins. Recombinant proteins are valuable means in biotechnological applications and are also emerging as alternatives in therapeutic applications. READ MORE

  5. 5. Emission of methane from northern lakes and ponds

    Author : Martin Wik; Patrick Crill; Isabelle Laurion; Stockholms universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; lakes; ponds; water bodies; methane; fluxes; ebullition; stable isotopes; arctic; subarctic; carbon cycling; climate change; geokemi; Geochemistry;

    Abstract : Northern lakes and ponds are abundant and emit large amounts of the potent climate forcer methane to the atmosphere at rates prone to change with amplified Arctic warming. In spite of being important, fluxes from surface waters are not well understood. READ MORE