Search for dissertations about: "insect cell cultures"

Showing result 1 - 5 of 7 swedish dissertations containing the words insect cell cultures.

2. 1. Physiological effects of conditioned medium and passage number on Spodoptera frugiperda Sf9 serum free cultures

   Author : Ingrid Svensson; Lena Häggström; Mats Martinelle; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Spodoptera frugiperda Sf9; insect cell cultures; conditioned medium; cell cycle phase dynamics; proteolytic activity; antibacterial activity; recombinant protein production; Bioteknik; Bioengineering; Bioteknik;

   Abstract : The aim of this study was to better understand the role of conditioned medium (CM) in Spodoptera frugiperda Sf9 insect cell proliferation and recombinant protein production using the baculovirus expression system. CM was found to stimulate cell proliferation. READ MORE

4. 2. Regulation of productivity in Trichoplusia ni and Spodoptera frugiperda Sf9 serum-free cultures

   Author : Karin Calles; Lena Häggström; Gunnel Dalhammar; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Insect cells; BEVS; Spodoptera frugiperda; Sf9; Trichoplusia ni; BTI-Tn-5B1-4; specific productivity; conditioned medium; conditioned medium factors; cell cycle; synchronization; G2 M; Bioteknik; Bioengineering; Bioteknik;

   Abstract : The aim of this work has been to characterize the effects of conditioned medium (CM) on insect cell productivity and physiology in order to get a better understanding about the mechanisms that regulate productivity in serum-free media. Two cell lines have been investigated, Spodoptera frugiperda (Sf9) and Trichoplusia ni (T. ni, BTI-Tn-5B1-4). READ MORE

5. 3. Impact of autocrine factors on physiology and productivity in Trichoplusia ni serum-free cultures

   Author : Ulrika Eriksson; Lena Häggström; Per Berglund; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Trichoplusia ni; High Five; insect cells; BEVS; serum-free medium; yeast extract; conditioned medium; autocrine regulation of proliferation; growth factors; cell cycle; metalloproteinase; specific productivity; Bioteknik; Bioengineering; Bioteknik;

   Abstract : The aim of this study was to increase the understanding of the mechanisms regulating cell proliferation and recombinant protein production in serum-free cultures of Trichoplusia ni (T. ni) insect cells.Conditioned medium (CM) was shown to contain both stimulatory and inhibitory factors (CM factors) influencing cell growth. READ MORE

6. 4. Physiology and productivity of serum-free Spodoptera frugiperda Sf9 insect cell cultures

   Author : Eva Lindskog; Lena Häggström; Spiros N. Agathos; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Animalcellteknologi; Bioengineering; Bioteknik;

   Abstract : The objective of this study was to investigate the mechanisms and factors controlling growth and proliferation in serum-free Spodoptera frugiperda Sf9 cultures as well as the implications of these factors for protein production in the baculovirus expression system. The physiology of recently thawed, low passage (Lp) Sf9 cultures, were compared to high passage (Hp) cultures at p>100. READ MORE

7. 5. Analysis of Genes and Proteins Involved in Wood Formation

   Author : Anders Winzell; Tuula Teeri; Alan Marchant; KTH; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Biokemi;

   Abstract : SAMMANFATTNING Vedbildning är en underliggande mekanism för så skilda områden som industri, boende och en hållbar miljö. Vedbildning sker i det vaskulära kambiet där organiserade celldelningar åtföljs av differentiering till specialiserade vedceller. READ MORE