Search for dissertations about: "isoenzymes"

Showing result 11 - 15 of 36 swedish dissertations containing the word isoenzymes.

  2. 11. Human α2,3-sialyltransferases. Structure and Function of Alternatively Spliced Transcripts

    Author : Ammi Grahn; Göteborgs universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; a2; 3-sialyltransferase; isotranscript; isoenzyme; alternative splicing; capillary electrophoresis;

    Abstract : The recruitment of human peripheral blood leukocytes to sites of infection and inflammation requires the surface expression of Sialyl Lewis x glycoconjugates (SLex) on white blood cells and their interaction with E- and P-selectins on activated endothelial cells. Human ST3Gal III, ST3Gal IV and ST3Gal VI genes code for a2,3?sialyltransferases potentially involved in the biosynthesis of the SLex epitope. READ MORE

  4. 12. Hydrolases as Catalysts for Green Chemistry and Industrial Applications - Esterase, Lipase and Phytase

    Author : Yasser Gaber; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; HPLC-EAT; sustainable environment; mutation; metallo-enzyme; phytate; b-propeller; biodegraqdable; metrics; green chemistry; Novozym 435; CalB; solvent-free; kinetic resolution; Biocatalysis; rational design; PLE; esterase; clopidogrel; enantioselectivity;

    Abstract : The use of enzymes in industrial applications has been recognised for providing clean processes with minimal impact on the environment. This thesis presents studies on engineering of enzymes and enzyme-based processes in the light of green chemistry and environmental sustainability, and focuses on three hydrolases: esterase, lipase and phytase. READ MORE

  5. 13. Studies on glyoxalase I from erythrocytes

    Author : Anne-Charlotte Aronsson; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES;

    Abstract : The aim of this investigation was to achieve an increased understanding of the properties of glyoxalase I, in order to eventually elucidate the physiological functions of the enzyme. Glyoxalase I was purified to homogeneity by a procedure involving affinity chromatography. READ MORE

  6. 14. Redesign of substrate specificity of glutathione transferase and glutathione reductase : Enzyme engineering by directed mutagenesis, phage-display selection and DNA shuffling

    Author : Lars O. Hansson; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Abstract : Human glutathione transferase (GST) A1-1 was expressed in fusion with the phage gene IIIproduct and a library of phage-displayed GST mutants was constructed by randomization of fouractive-site residues. Variant GSTs were isolated by mechanism-based phage display selection, using an immobilized transition-state analog of a nucleophilic aromatic substitution (SNAr) reaction. READ MORE

  7. 15. Discovery of fiber-active enzymes in Populus wood

    Author : Henrik Aspeborg; KTH; []
    Keywords : Populus; xylogenesis; secondary cell wall; cellulose; hemicellulose; microarrays; transcript profiling; carbohydrate-active enzyme; glycosyltransferase; glycoside hydrolase;

    Abstract : Renewable fibers produced by forest trees provide excellentraw material of high economic value for industrialapplications. Despite this, the genes and corresponding enzymesinvolved in wood fiber biosynthesis in trees are poorlycharacterized. READ MORE