Search for dissertations about: "isolation of enzyme"

Showing result 1 - 5 of 44 swedish dissertations containing the words isolation of enzyme.

2. 1. Standardization of Islet Isolation and Transplantation Variables

   Author : Andrew S Friberg; Olle Korsgren; Daniel Brandhorst; Paul R. V. Johnson; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Islet isolation; standardization; enzyme; gradient separation; digital imaging analysis; DIA; transplantation outcome; islet transplantation; prediction; Medical technology; Medicinsk teknik; Medical Cell Biology; Medicinsk cellbiologi; Computerized Image Processing; Datoriserad bildbehandling;

   Abstract : Currently, the transplantation of islets of Langerhans is a viable means to maintain control of blood sugar levels and reduce the risk of hypoglycemia in defined populations with brittle type I diabetes mellitus or those requiring pancreatectomy. However, the process of islet isolation is highly variable and not all isolations result in islet numbers or quality suitable for transplantation. READ MORE

4. 2. Studies on Cytotoxic and Neutrophil Challenging Polypeptides and Cardiac Glycosides of Plant Origin

   Author : Senia Johansson; Tore Mellstrand; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmaceutical chemistry; cytotoxic; neutrophil; polypeptides; Phoradendron tomentosum; Digitalis purpurea; plant; mistletoe; MS MS; Edman degradation; breast cancer; macrocyclic peptides; multidrug resistance protein; primary structures; phoratoxin; isolation; digitoxin; cardiac lycoside; structure-activity; Farmaceutisk kemi; Pharmaceutical chemistry; Farmaceutisk kemi; Pharmacognosy; farmakognosi;

   Abstract : This thesis examines the isolation and characterisation (biological and chemical) of polypeptides from plants. A fractionation protocol was developed and applied on 100 plant materials with the aim of isolating highly purified polypeptide fractions from small amounts of plant materials. READ MORE

5. 3. F F₁-ATPase of plant mitochondria : purification and characterization of the structural and functional properties of the individual subunits

   Author : Beston Hamasur; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES;

   Abstract : Plant leaf cells constitute a unique system in which both mitochondria and chloroplasts are responsible for energy conversion. In both organelles, ATP synthase is responsible for the synthesis of ATP from ADP and Pi in a process coupled to an electron transfer chain. READ MORE

6. 4. Mass Spectrometric Analysis of Oxylipins : Application to Cytochrome P450-Dependent Metabolism

   Author : Tomas Nilsson; Ernst Oliw; Johan Bylund; Mikael Hedeland; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; epoxyalcohols; fatty acids; hydroperoxides; electrospray ionization; ichthyosis; CYP4; linear ion trap; fragmentation mechanism; Pharmaceutical pharmacology; Farmaceutisk farmakologi; Biochemical Pharmacology; Biokemisk farmakologi;

   Abstract : Cytochrome P450 (CYP) family 4 constitutes monoxygenases responsible for hydroxylation of fatty acids and other lipids. For example, CYP4F3 metabolizes leukotrienes and CYP4F8 prostaglandin H. Importantly, six of the twelve CYP4 enzymes are orphans, i.e. READ MORE

7. 5. Technical challenges in human islet isolation

   Author : Magnus Ståhle; Olle Korsgren; Andrew Friberg; Federico Bertuzzi; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES;

   Abstract : Transplantation of islets of Langerhans is an effective treatment option for patients with brittle type 1 diabetes mellitus. This treatment restores glucose control and also reduces hypoglycemia. Unfortunately, the outcome from islet isolations is variable, and many preparations do not yield sufficient islet number or islet quality. READ MORE