Search for dissertations about: "isothermal titration calorimetry"

Showing result 11 - 15 of 36 swedish dissertations containing the words isothermal titration calorimetry.

  2. 11. Development of Fluorescent Nucleobase Analogues - Intrinsically labelled nucleic acids for molecular binding investigations

    Author : Mattias Bood; Göteborgs universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; DNA; RNA; Fluorescent nucleobase analogue; FRET; pre-microRNA; isothermal titration calorimetry; surface plasmon resonance; aminoglycoside; binding interaction;

    Abstract : This thesis focuses on the design, synthesis and utilization of fluorescent nucleobase analogues (FBAs). FBAs are an important class of compounds, used in the research of nucleic acids. The class of canonical FBAs, i.e. READ MORE

  4. 12. Monolithic separation media synthesized in capillaries and their applications for molecularly imprinted networks

    Author : Julien Courtois; Knut Irgum; Ruth Freitag; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; bupivacaine; etching; phosphorylated tyrosine; poly ethylene glycol ; silanization; transmission electron microscopy; fused silica capillaries; isothermal titration calorimetry; local anæsthetic; molecularly imprinted polymer; monolith; nuclear magnetic resonance; Analytical chemistry; Analytisk kemi;

    Abstract : The thesis describes the synthesis of chromatographic media using several different approaches, their characterizations and applications in liquid chromatography. The steps to achieve a separation column for a specific analyte are presented. The main focus of the study was the design of novel molecularly imprinted polymers. READ MORE

  5. 13. Biophysical studies of DNA binding – by the large filament-forming protein Rad51 and the small minor-groove binder Hoechst 33258

    Author : Louise Fornander; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Rad51; Linear Dichroism; DNA; Isothermal Titration Calorimetry.; Spectroscopy; Nanofluidics; Hoechst 33258; DNA-binding ligands;

    Abstract : Mechanistic insight into the nature of DNA-binding ligands is crucial for both drug development as well as understanding more complex biological reactions that take place in the cell. In this Thesis, two rather different DNA-binding molecules are considered: 1. READ MORE

  6. 14. Cellular Dyes and Bioactive Peptides - Cell Membrane Interactions and Cellular Uptake

    Author : Maria Matson Dzebo; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Membrane Interactions; Spectroscopy; Cell-Penetrating Peptides; Bioactive Peptides; Cell Membrane; Confocal Laser Scanning Microscopy; Cellular Uptake; AF-16; Voltage-Sensitive Dyes; Cell Dyes; Antisecretory Factor; Ruthenium Complexes; Isothermal Titration Calorimetry; Flow-Cytometry;

    Abstract : Knowledge about mechanisms behind interactions of molecules with biomembranes and cellular uptake is very important for understanding biological processes and for drug design. The work described in this thesis has focused on interactions of cellular dyes and bioactive peptides with cells and the cell membrane. READ MORE

  7. 15. Structure determination and thermodynamic stabilization of an engineered protein-protein complex

    Author : Elisabet Wahlberg; Per-Åke Nygren; Bengt-Harald Jonsson; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; protein structure; coupled folding; NMR spectroscopy; protein stability; protein-protein interactions; binding thermodynamics; isothermal titration calorimetry; protein engineering; Structural biochemistry; Strukturbiokemi;

    Abstract : The interaction between two 6 kDa proteins has been investigated. The studied complex of micromolar affinity (Kd) consists of the Z domain derived from staphylococcal protein A and the related protein ZSPA-1, belonging to a group of binding proteins denoted affibody molecules generated via combinatorial engineering of the Z domain. READ MORE