Search for dissertations about: "kinetic enzyme inhibition"

Showing result 1 - 5 of 44 swedish dissertations containing the words kinetic enzyme inhibition.

2. 1. Sensitivity, Noise and Detection of Enzyme Inhibition in Progress Curves

   Author : Omar Gutierrez Arenas; Helena Danielson; Athel Cornish-Bowden; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Progress curves; limit of detection; Z -factor; primary screening; enzyme inhibition; error structure; sensitivity analysis; High-throughput screning; Biokemi;

   Abstract : Starting with the development of an enzymatic assay, where an enzyme in solution hydrolysed a solid-phase bound peptide, a model for the kinetics of enzyme action was introduced. This model allowed the estimation of kinetic parameters and enzyme activity for a system that has the peculiarity of not being saturable with the substrate, but with the enzyme. READ MORE

4. 2. Kinetic studies of NS3 and NS5B from Hepatitis C virus : Implications and applications for drug discovery

   Author : Göran Dahl; Helena Danielson; Raffaele De Francesco; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Hepatitis C virus; NS3; NS5B; enzyme kinetics; inhibition; resistance; drug; Biochemistry; Biokemi; Biokemi; Biochemistry;

   Abstract : The aim of these studies was to increase our understanding of the non-structural proteins 3 and 5B (NS3 and NS5B) from the hepatitis C virus (HCV), and thereby contribute to the development of new and better drugs against HCV.By studying NS3 with substitutions identified to be associated with resistance to NS3 inhibitors in clinical trials (R155Q, A156T and D168V) it was found that not all inhibitors were affected, indicating that cross-resistance can be avoided. READ MORE

5. 3. Chemoenzymatic Resolution in Dynamic Systems : Screening, Classification and Asymmetric Synthesis

   Author : Yan Zhang; Olof Ramström; Sabine Flitsch; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; constitutional dynamic chemistry; dynamic systemic resolution; dynamic kinetic resolution; enzyme catalysis; transesterification; enzyme promiscuity; asymmetric synthesis; pattern recognition; self-inhibition.;

   Abstract : This  thesis  is  divided  into  four  parts,  all  centered  around  Constitutional Dynamic  Chemistry  (CDC)  and  Dynamic  Kinetic  Resolution  (DKR)  using biocatalysts for selective transformations, and their applications in screening of bioactive compounds, organic synthesis, and enzyme classification.   In  part  one,  an  introduction  to  CDC  and  DKR  is  presented,  illustrating  the basic  concepts,  practical  considerations  and  potential  applications  of  such dynamic systems, thus providing the background information for the studies in the following chapters. READ MORE

6. 4. Oxidation of 1,2-Diols Using Alcohol Dehydrogenases : From Kinetic Characterization to Directed Evolution

   Author : Cecilia Blikstad; Mikael Widersten; Stefan Lutz; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; enzyme kinetics; alcohol dehydrogenase; directed evolution; enzyme engineering; diol; α-hydroxy aldehyde; Biokemi; Biochemistry;

   Abstract : The use of enzymes as catalysts for chemical transformations has emerged as a “greener” alternative to traditional organic synthesis. An issue to solve though, is that enzymes are designed by nature to catalyze reactions in a living cell and therefore, in many cases, do not meet the requirements of a suitable biocatalyst. READ MORE

7. 5. Protease Activity, Inhibition and Ligand Interaction Analysis : Developments and Applications for Drug Discovery

   Author : Thomas Gossas; Helena Danielson; Mikael Widersten; Alexander Wlodawer; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; proteases; kinetics; biosensors; inhibition; calcium; drug discovery; Biokemi;

   Abstract : The present study has focused on characterising protease-ligand interactions in the context of drug discovery. The proteases that have been studied are human matrix metallopeptidase 12 (MMP-12), HIV-protease and Hepatitis C virus (HCV) NS3/NS4A protease. READ MORE