Search for dissertations about: "lehtiö"

Showing result 1 - 5 of 6 swedish dissertations containing the word lehtiö.

  2. 1. Functional studies and engineering of family 1 carbohydrate-binding modules

    Author : Janne Lehtiö; KTH; []
    Keywords : Cellulose-binding; carbohydrate-binding modules; phage display; bacterial surface display; combinatorial protein library; protein engineering; Trichoderma reesei; Staphylococcus carnosus;

    Abstract : The family 1 cellulose-binding modules (CBM1) form a groupof small, stable carbohydrate-binding proteins. These modulesare essential for fungal cellulosedegradation. This thesisdescribes both functional studies of the CBM1s as well asprotein engineering of the modules for several objectives. READ MORE

  4. 2. Molecular mechanisms underlying the activation of ALC1 nucleosome remodeling

    Author : Luka Bacic; Sebastian Deindl; Stefan D. Knight; Lari Lehtiö; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; ALC1; CHD1L; nucleosome; ADP-ribosylation; chromatin; remodeling; PARP1; HPF1; DNA damage; Biology with specialization in Structural Biology; Biologi med inriktning mot strukturbiologi; Biology with specialization in Structural Biology; Biologi med inriktning mot strukturbiologi; Biology with specialization in Structural Biology; Biologi med inriktning mot strukturbiologi;

    Abstract : Packaging DNA into chromatin represses essential DNA-based processes, such as transcription, DNA replication, and repair. To change the accessibility of DNA, cells have evolved a set of enzymes referred to as chromatin remodelers that act on the basic repeat unit of chromatin,  the nucleosome. READ MORE

  5. 3. Development and Application of Proximity Assays for Proteome Analysis in Medicine

    Author : Felipe Marques Souza de Oliveira; Masood Kamali-Moghaddam; Ulf Landegren; Mats Gullberg; Janne Lehtiö; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Solid-phase proximity ligation assay; post-translational modifications; glycosylation; phosphorylation; Enzyme-linked immunosorbent assay; immunoassay and rolling circle amplification; Proximity Extension Assay; inflammation protein biomarkers; autoantibodies; autoimmune disease;

    Abstract : Along with proteins, a myriad of different molecular biomarkers, such as post-translational modifications and autoantibodies, could be used in an attempt to improve disease detection and progression. In this thesis, I build on several iterations of the proximity ligation assay to develop and apply new adaptable methods to facilitate detection of proteins, autoantibodies and post-translational modifications. READ MORE

  6. 4. Molecular Signatures of Neuropathic Pain : Revealing Pain-Related Signaling Processes in Spinal Cord Using Mass Spectrometric Methodologies

    Author : Ping Sui; Jonas Bergquist; Konstantin Artemenko; Janne Lehtiö; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Mass spectrometry; Spinal cord; Neuropathic pain; Quantitative proteomics; Imaging mass spectrometry; Kemi med inriktning mot analytisk kemi; Chemistry with specialization in Analytical Chemistry;

    Abstract : In this thesis, the detection of global proteomics alteration and changes in neuropeptide distribution caused by neuropathic pain in rat spinal cord tissue was the main focus. Neuropathic pain (NP) is a major clinical syndrome caused by disease or dysfunction of the nervous system and often mediated by neuronal networks in the spinal cord. READ MORE

  7. 5. Multivariate profiling of metabolites in human disease : Method evaluation and application to prostate cancer

    Author : Elin Thysell; Henrik Antti; Janne Lehtiö; Umeå universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; metabolite profiling; metabolomics; predictive metabolomics; mass spectrometry; GC-MS; biomarkers; chemometrics; design of experiments; multivariate data analysis; prostate cancer; bone metastases; plasma; biologisk kemi; biological chemistry;

    Abstract : There is an ever increasing need of new technologies for identification of molecular markers for early diagnosis of fatal diseases to allow efficient treatment. In addition, there is great value in finding patterns of metabolites, proteins or genes altered in relation to specific disease conditions to gain a deeper understanding of the underlying mechanisms of disease development. READ MORE