Search for dissertations about: "library preparation"

Showing result 1 - 5 of 34 swedish dissertations containing the words library preparation.

  1. 1. Library Preparation for High Throughput DNA Sequencing

    Author : Anders Jemt; Joakim Lundeberg; Petri Auvinen; KTH; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; DNA; RNA; sequencing; massively parallel sequencing; library preparation; automation; genome; transcriptome; Biotechnology; Bioteknologi;

    Abstract : Order 3 billion base pairs of DNA in the correct order and you get the blueprint of a human, the genome. Before the introduction of massively parallel sequencing a little more than a decade ago it would cost around $10 million to get this blueprint. READ MORE

  2. 2. Methods to Prepare DNA for Efficient Massive Sequencing

    Author : Sverker Lundin; Joakim Lundeberg; Harold Swerdlow; KTH; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; TEKNIK OCH TEKNOLOGIER; MEDICIN OCH HÄLSOVETENSKAP; ENGINEERING AND TECHNOLOGY; MEDICAL AND HEALTH SCIENCES; DNA; Massive sequencing; Next Generation Sequencing; Library Preparation; Barcoding; Multiplexing;

    Abstract : Massive sequencing has transformed the field of genome biology due to the continuous introduction and evolution of new methods. In recent years, the technologies available to read through genomes have undergone an unprecedented rate of development in terms of cost-reduction. READ MORE

  3. 3. Advances in DNA Detection

    Author : Yajing Song; Olof Emanuelsson; Jonas Tegenfeldt; KTH; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; DNA detection; active filter paper; visual detection; throughput; fluorescence; superparamagnetic beads; rare DNA; Q-PCR; chemical fragmentation; DNA library preparation; massively parallel sequencing; SRA - Molekylär biovetenskap; SRA - Molecular Bioscience;

    Abstract : DNA detection technologies have an increasing importance in our everyday lives, with applications ranging from microbial diagnostics to forensic analysis, food safety evaluation, and environmental monitoring. Currently, as the associated costs decrease, DNA diagnostic techniques are routinely used in research laboratories, in clinical and forensic practice. READ MORE

  4. 4. Simplified Routines for Sample Preparation and Analysis of Chemical Warfare Agent Degradation Products

    Author : Raja Subramaniam; Calle Nilsson; Paula Vanninen; Umeå universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; Nerve agents; Alkyl alkylphosphonic acids; Solid phase derivatization; Fluorinated diazomethane reagent; High-energy collision induced dissociation; CID MS MS library; analytisk kemi; Analytical Chemistry;

    Abstract : The thesis describes the development of new and improved methods for analyzing degradation markers from organophosphorus Chemical Warfare Agents (CWAs). Paper I and II describes an innovative and significantly improved method for the enrichment, derivatization (trimethysilylation) and GC-MS analysis of a broad range of organophosphorus CWAs degradation markers, namely the alkylphosphonic acids and a zwitterionic compound. READ MORE

  5. 5. Science mapping and research evaluation : a novel methodology for creating normalized citation indicators and estimating their stability

    Author : Cristian Colliander; Rickard Danell; Olle Persson; Jesper Schneider; Umeå universitet; []
    Keywords : SOCIAL SCIENCES; SAMHÄLLSVETENSKAP; SOCIAL SCIENCES; SAMHÄLLSVETENSKAP; SAMHÄLLSVETENSKAP; SAMHÄLLSVETENSKAP; SOCIAL SCIENCES; SOCIAL SCIENCES; document-document similarity; science mapping; citation analysis; citation normalization; stability analysis; citation impact; research evaluation; library and information science; biblioteks- och informationsvetenskap;

    Abstract : The purpose of this thesis is to contribute to the methodology at the intersection of relational and evaluative bibliometrics. Experimental investigations are presented that address the question of how we can most successfully produce estimates of the subject similarity between documents. READ MORE